Sebaceous neoplasms: prevalence of HPV infection and relation to immunohistochemical surrogate markers

Maelle Saliba; Muhammad Shaheen; Rana El Hajj; Fatmeh Ibrahim Abbas; Shaarif Bashir; Umer Nisar Nisar Sheikh; Rami Mahfouz; Asif Loya; Ibrahim Khalifeh

doi:10.1684/ejd.2021.4008

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Sebaceous neoplasms: prevalence of HPV infection and relation to immunohistochemical surrogate markers

Journal article

Peer reviewed

Sebaceous neoplasms: prevalence of HPV infection and relation to immunohistochemical surrogate markers

Maelle Saliba, Muhammad Shaheen, Rana El Hajj, Fatmeh Ibrahim Abbas, Shaarif Bashir, Umer Nisar Nisar Sheikh, Rami Mahfouz, Asif Loya and Ibrahim Khalifeh

EJD. European journal of dermatology, Vol.31(2), pp.170-175

04/01/2021

DOI: 10.1684/ejd.2021.4008

PMID: 34001468

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Abstract

Background Sebaceous neoplasms (SNs) and carcinomas (SCs) represent rare skin adnexal tumours. Objectives To establish the prevalence of HPV in SNs, assess the relationship between HPV, p16 and p53 expression, and further elucidate the carcinogenetic course of SCs. Materials & Methods A total of 113 resected SNs (five sebaceous adenomas, 10 sebaceomas and 98 SCs) from the Near-East were reviewed. Clinical information (age, gender, size and anatomical location), microscopic variables, and expression of several immunohistochemical markers (EMA, CK5/6, p63, p40, AR, p16 and p53) were documented. Cases were evaluated by fluorescently labelled PCR for HPV detection, followed by DNA microarray hybridization for subtype detection. Results HPV infection was detected in 9.4% of SNs: 28.6% sebaceous adenomas (HPV-16 and HPV-66), 9.1% sebaceomas (HPV-18) and 8.1% SCs. High-risk HPV types (HPV-16, -18, -52 and -66) constituted 90.9% of HPV infections. Histologically, HPV-positive SCs showed significantly milder cytologic atypia and patchy cellular necrosis. p16 was expressed in SNs irrespective of HPV status (20.0%, 33.3% and 65.5% of HPV-negative sebaceous adenomas, sebaceomas, and SCs, respectively), and p53 was abnormally expressed in 95.5% of HPV-negative SCs and all HPV-positive SCs. Conclusion HPV infection is significantly present in benign and malignant SNs. HPV-positive SCs exhibit less cytologic atypia and necrosis than HPV-negative cases. p16 is not a surrogate marker of HPV infection in the SN setting. Further elucidation of various carcinogenic mechanisms in SCs will allow clinicians to single out the various populations at risk, optimize possible preventive strategies and develop targeted therapies

Dermatology

Life Sciences & Biomedicine

Science & Technology

Details

Title: Subtitle: Sebaceous neoplasms: prevalence of HPV infection and relation to immunohistochemical surrogate markers
Creators: Maelle Saliba - American University of Beirut Medical Center
Muhammad Shaheen - American University of Beirut Medical Center
Rana El Hajj - American University of Beirut Medical Center
Fatmeh Ibrahim Abbas - American University of Beirut Medical Center
Shaarif Bashir - Shaukat Khanum Memorial Cancer Hospital and Research Center
Umer Nisar Nisar Sheikh - Shaukat Khanum Mem Canc Hosp & Res Ctr, Dept Pathol, Lahore, Pakistan
Rami Mahfouz - American University of Beirut Medical Center
Asif Loya - Shaukat Khanum Memorial Cancer Hospital and Research Center
Ibrahim Khalifeh - American University of Beirut Medical Center
Resource Type: Journal article
Publication Details: EJD. European journal of dermatology, Vol.31(2), pp.170-175
DOI: 10.1684/ejd.2021.4008
PMID: 34001468
NLM abbreviation: Eur J Dermatol
ISSN: 1167-1122
eISSN: 1952-4013
Publisher: JLE
Number of pages: 6
Language: English
Date published: 04/01/2021
Academic Unit: Pathology
Record Identifier: 9985217818202771

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