Journal article
Sec6/8 Complex Is Recruited to Cell–Cell Contacts and Specifies Transport Vesicle Delivery to the Basal-Lateral Membrane in Epithelial Cells
Cell (Cambridge), Vol.93(5), pp.731-740
1998
DOI: 10.1016/S0092-8674(00)81435-X
PMID: 9630218
Abstract
In budding yeast, the Sec6/8p complex is essential for generating cell polarity by specifying vesicle delivery to the bud tip. We show that Sec6/8 homologs are components of a cytosolic, ∼17S complex in nonpolarized MDCK epithelial cells. Upon initiation of calcium-dependent cell–cell adhesion, ∼70% of Sec6/8 is rapidly (t
1/2 ≈ 3–6 hr) recruited to sites of cell–cell contact. In streptolysin-O-permeabilized MDCK cells, Sec8 antibodies inhibit delivery of LDL receptor to the basal-lateral membrane, but not p75
NTR to the apical membrane. These results indicate that lateral membrane recruitment of the Sec6/8 complex is a consequence of cell–cell adhesion and is essential for the biogenesis of epithelial cell surface polarity.
Details
- Title: Subtitle
- Sec6/8 Complex Is Recruited to Cell–Cell Contacts and Specifies Transport Vesicle Delivery to the Basal-Lateral Membrane in Epithelial Cells
- Creators
- Kent K Grindstaff - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5345, USACharles Yeaman - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5345, USANiroshana Anandasabapathy - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5345, USAShu-Chan Hsu - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5345, USAEnrique Rodriguez-Boulan - Dyson Vision Research Institute and, Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021, USARichard H Scheller - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5345, USAW.James Nelson - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5345, USA
- Resource Type
- Journal article
- Publication Details
- Cell (Cambridge), Vol.93(5), pp.731-740
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0092-8674(00)81435-X
- PMID
- 9630218
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Language
- English
- Date published
- 1998
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025437402771
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