Segregation of genomes in polyploid tumour cells following mitotic catastrophe

Jekaterina Erenpreisa; M Kalejs; F Ianzini; E.A Kosmacek; M.A Mackey; D Emzinsh; M.S Cragg; A Ivanov; T.M Illidge

doi:10.1016/j.cellbi.2005.10.008

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Segregation of genomes in polyploid tumour cells following mitotic catastrophe

Journal article

Peer reviewed

Segregation of genomes in polyploid tumour cells following mitotic catastrophe

Jekaterina Erenpreisa, M Kalejs, F Ianzini, E.A Kosmacek, M.A Mackey, D Emzinsh, M.S Cragg, A Ivanov and T.M Illidge

Cell biology international, Vol.29(12), pp.1005-1011

2005

DOI: 10.1016/j.cellbi.2005.10.008

PMID: 16314119

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Abstract

Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.

Mitotic catastrophe

Resuming of mitosis

Link to microtubule-organising centre

De-polyploidisation

Radial arrangement of genomes

Details

Title: Subtitle: Segregation of genomes in polyploid tumour cells following mitotic catastrophe
Creators: Jekaterina Erenpreisa - Biomedicine Centre of the Latvia University, Ratsupites 1, Riga LV-1067, Latvia
M Kalejs - Biomedicine Centre of the Latvia University, Ratsupites 1, Riga LV-1067, Latvia
F Ianzini - Department Pathology, University of Iowa, Iowa City, IA, USA
E.A Kosmacek - Department Biomedical Engineering, University of Iowa, Iowa City, IA, USA
M.A Mackey - Department Pathology, University of Iowa, Iowa City, IA, USA
D Emzinsh - Oncology Centre of Latvia, Riga, Latvia
M.S Cragg - University of Southampton, Southampton, UK
A Ivanov - Biomedicine Centre of the Latvia University, Ratsupites 1, Riga LV-1067, Latvia
T.M Illidge - Paterson Cancer Research Institute, Manchester, UK
Resource Type: Journal article
Publication Details: Cell biology international, Vol.29(12), pp.1005-1011
DOI: 10.1016/j.cellbi.2005.10.008
PMID: 16314119
NLM abbreviation: Cell Biol Int
ISSN: 1065-6995
eISSN: 1095-8355
Publisher: Elsevier Ltd
Language: English
Date published: 2005
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Radiology
Record Identifier: 9984064585402771

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