Selected contribution: Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress

G P Lambert; C V Gisolfi; D J Berg; P L Moseley; L W Oberley; K C Kregel

doi:10.1152/japplphysiol.00787.2001

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Selected contribution: Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress

Journal article

Peer reviewed

Selected contribution: Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress

G P Lambert, C V Gisolfi, D J Berg, P L Moseley, L W Oberley and K C Kregel

Journal of applied physiology (1985), Vol.92(4), pp.1750-1761

04/2002

DOI: 10.1152/japplphysiol.00787.2001

PMID: 11896046

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Abstract

The purpose of this study was to characterize intestinal permeability changes over a range of physiologically relevant body temperatures in vivo and in vitro. Initially, FITC-dextran (4,000 Da), a large fluorescent molecule, was loaded into the small intestine of anesthetized rats. The rats were then maintained at approximately 37 degrees C or heated over 90 min to a core body temperature of approximately 41, approximately 41.5, or approximately 42.5 degrees C. Permeability was greater in the 42.5 degrees C group compared with the 37, 41, or 41.5 degrees C groups. Histological analysis revealed intestinal epithelial damage in heated groups. Everted intestinal sacs were then used to further characterize hyperthermia-induced intestinal permeability and to study the potential role of oxidative and nitrosative stress. Increased permeability to 4,000-Da FITC-dextran in both small intestinal and colonic sacs was observed at a temperature of 41.5-42 degrees C compared with 37 degrees C, along with widespread intestinal epithelial damage. Administration of antioxidant enzyme mimics or a nitric oxide synthase inhibitor did not reduce permeability due to heat stress, and tissue concentrations of a lipid peroxidation product were not altered by heat stress, suggesting that oxidative and nitrosative stress were not likely mediators of this phenomenon in vitro. In conclusion, hyperthermia produced increased permeability and marked intestinal epithelial damage both in vivo and in vitro, suggesting that thermal disruption of epithelial membranes contributes to the intestinal barrier dysfunction manifested with heat stress.

Intestinal Mucosa - metabolism

Fluorescein-5-isothiocyanate - pharmacokinetics

Intestinal Mucosa - ultrastructure

Fever - metabolism

Oxidative Stress - physiology

Rats

Fluorescein-5-isothiocyanate - analogs & derivatives

Male

Microscopy, Electron

Rats, Sprague-Dawley

Fever - physiopathology

Dextrans - pharmacokinetics

Animals

Cell Membrane Permeability - physiology

Free Radicals - metabolism

In Vitro Techniques

Nitric Oxide - metabolism

Intestinal Absorption - physiology

Details

Title: Subtitle: Selected contribution: Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress
Creators: G P Lambert - Department of Exercise Science, The University of Iowa, Iowa City, Iowa 52242-1111, USA
C V Gisolfi
D J Berg
P L Moseley - University of New Mexico
L W Oberley
K C Kregel
Resource Type: Journal article
Publication Details: Journal of applied physiology (1985), Vol.92(4), pp.1750-1761
DOI: 10.1152/japplphysiol.00787.2001
PMID: 11896046
NLM abbreviation: J Appl Physiol (1985)
ISSN: 8750-7587
eISSN: 1522-1601
Publisher: United States
Grant note: AG-12350 / NIA NIH HHS AG-14687 / NIA NIH HHS AR-40771 / NIAMS NIH HHS HL-61389 / NHLBI NIH HHS
Language: English
Date published: 04/2002
Academic Unit: Radiation Oncology; Provost Office Administration; Health, Sport, and Human Physiology ; Internal Medicine
Record Identifier: 9983979997202771

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