Journal article
Selective accumulation of the complement membrane attack complex in aging choriocapillaris
Experimental Eye Research, Vol.146, pp.393-397
05/2016
DOI: 10.1016/j.exer.2015.09.003
PMCID: PMC4788569
PMID: 26368849
Abstract
The complement membrane attack complex (MAC) shows increased abundance in the choriocapillaris during normal aging and is especially prevalent in age-related macular degeneration (AMD). While perivascular MAC accumulation occurs in the choroid, it is not well understood whether similar deposition occurs in other aging tissues. In this study we examined the abundance of MAC across multiple human tissues. For studies on fixed tissues, paraffin sections were obtained from six human donor eyes and a commercially available tissue array containing 19 different tissues. Immunohistochemical labeling was performed using antibodies directed against the MAC and intercellular adhesion molecule-1 (ICAM-1), as well as the lectin Ulex europaeus agglutinin-I (UEA-I). The choriocapillaris was the only tissue with high levels of the MAC, which was not detected in any of the 38 additional samples from 19 tissues. ICAM-1 was abundantly expressed in the majority of tissues evaluated, and UEA-I labeled the vasculature in all tissues. A second experiment was performed using unfixed frozen sections of RPE-choroid and 7 extraocular tissues, which confirmed the relatively limited localization of the MAC to the choriocapillaris. In comparison to other tissues assessed, the restricted accumulation of MAC in the choriocapillaris may, in part, explain the specificity of AMD to the neural retina, RPE and choroid, and the relative absence of systemic pathology in this disease. •AMD is a degenerative disease with increased activation of complement in the choriocapillaris.•The membrane attack complex was evaluated in human choroid and 19 other tissues.•The labeling pattern observed for the membrane attack complex suggests the choroid is a hot spot for its deposition.
Details
- Title: Subtitle
- Selective accumulation of the complement membrane attack complex in aging choriocapillaris
- Creators
- Kathleen R ChircoBudd A TuckerEdwin M StoneRobert F Mullins
- Resource Type
- Journal article
- Publication Details
- Experimental Eye Research, Vol.146, pp.393-397
- DOI
- 10.1016/j.exer.2015.09.003
- PMID
- 26368849
- PMCID
- PMC4788569
- NLM abbreviation
- Exp Eye Res
- ISSN
- 0014-4835
- eISSN
- 1096-0007
- Publisher
- Elsevier BV
- Grant note
- Ronald and Annette Massman Choroideremia Research Fund Martin and Ruth Carver Chair in Ocular Cell Biology Elmer and Sylvia Sramek Charitable Foundation EY-024605 / NIH (http://dx.doi.org/10.13039/100000002)
- Language
- English
- Date published
- 05/2016
- Academic Unit
- Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980093902771
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