Selective effects of calcium chelators on anterograde and retrograde protein transport in the cell

Ji-Long Chen; Jatinder P Ahluwalia; Mark Stamnes

doi:10.1074/jbc.M204157200

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Selective effects of calcium chelators on anterograde and retrograde protein transport in the cell

Journal article

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Selective effects of calcium chelators on anterograde and retrograde protein transport in the cell

Ji-Long Chen, Jatinder P Ahluwalia and Mark Stamnes

The Journal of biological chemistry, Vol.277(38), pp.35682-35687

09/20/2002

DOI: 10.1074/jbc.M204157200

PMID: 12114519

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Abstract

Calcium plays a regulatory role in several aspects of protein trafficking in the cell. Both vesicle fusion and vesicle formation can be inhibited by the addition of calcium chelators. Because the effects of calcium chelators have been studied predominantly in cell-free systems, it is not clear exactly which transport steps in the secretory pathway are sensitive to calcium levels. In this regard, we have studied the effects of calcium chelators on both anterograde and retrograde protein transport in whole cells. Using both cytochemical and biochemical analyses, we find that the anterograde-directed exit of vesicular stomatitis virus G protein and the retrograde-directed exit of Shiga toxin from the Golgi apparatus are both inhibited by calcium chelation. The exit of vesicular stomatitis virus G from a pre-Golgi compartment and the exit of Shiga toxin from an endosomal compartment are sensitive to the membrane-permeant calcium chelator 1,2-bis(2-amino phenoxy)ethane-N,N,N',N'-tetraacetic acid-tetrakis (acetoxymethyl ester) (BAPTA-AM). By contrast, endoplasmic reticulum exit and endocytic internalization from the plasma membrane are not affected by BAPTA. Together, our data show that some, but not all, trafficking steps in the cell may be regulated by calcium. These studies provide a framework for a more detailed analysis of the role of calcium as a regulatory agent during protein transport.

Base Sequence

Calcium - metabolism

Chelating Agents - metabolism

DNA Primers

Egtazic Acid - analogs & derivatives

Egtazic Acid - metabolism

Endoplasmic Reticulum - metabolism

Golgi Apparatus - metabolism

Membrane Glycoproteins

Protein Transport

Viral Envelope Proteins - metabolism

Details

Title: Subtitle: Selective effects of calcium chelators on anterograde and retrograde protein transport in the cell
Creators: Ji-Long Chen - University of Iowa
Jatinder P Ahluwalia - University of Iowa
Mark Stamnes - University of Iowa
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.277(38), pp.35682-35687
DOI: 10.1074/jbc.M204157200
PMID: 12114519
ISSN: 0021-9258
eISSN: 1083-351X
Language: English
Date published: 09/20/2002
Academic Unit: Molecular Physiology and Biophysics; Internal Medicine
Record Identifier: 9984297596502771

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