Journal article
Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment
Bioresource technology, Vol.203, pp.80-88
03/01/2016
DOI: 10.1016/j.biortech.2015.12.012
PMID: 26722806
Abstract
The synergistic effect of temperature (25-65 °C) and total specific energy input (0.55-1.11 kWh kgDW(-1)) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55 °C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55 °C. Above 55 °C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).
Details
- Title: Subtitle
- Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment
- Creators
- P R Postma - Wageningen University & ResearchG Pataro - University of SalernoM Capitoli - University of SalernoM J Barbosa - Wageningen University & ResearchR H Wijffels - Wageningen University & ResearchM H M Eppink - Wageningen University & ResearchG Olivieri - Wageningen University & ResearchG Ferrari - University of Salerno
- Resource Type
- Journal article
- Publication Details
- Bioresource technology, Vol.203, pp.80-88
- DOI
- 10.1016/j.biortech.2015.12.012
- PMID
- 26722806
- NLM abbreviation
- Bioresour Technol
- ISSN
- 0960-8524
- eISSN
- 1873-2976
- Grant note
- DOI: 10.13039/501100001830, name: Wageningen University and Research Centre
- Language
- English
- Date published
- 03/01/2016
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984398028702771
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