Journal article
Selective gene expression analysis of muscular and vascular components in hearts using laser microdissection method
International journal of vascular medicine, Vol.2012(2012), pp.420-424
01/01/2012
DOI: 10.1155/2012/863410
PMCID: PMC3384972
PMID: 22778964
Abstract
Background. The heart consists of various kinds of cell components. However, it has not been feasible to separately analyze the gene expression of individual components. The laser microdissection (LMD) method, a new technology to collect target cells from the microscopic regions, has been used for malignancies. We sought to establish a method to selectively collect the muscular and vascular regions from the heart sections and to compare the marker gene expressions with this method. Methods and Results. Frozen left ventricle sections were obtained from Wistar-Kyoto rats (WKY) and stroke-prone spontaneously hypertensive rats (SHR-SP) at 24 weeks of age. Using the LMD method, the muscular and vascular regions were selectively collected under microscopic guidance. Real-time RT-PCR analysis showed that brain-type natriuretic peptide (BNP), a marker of cardiac myocytes, was expressed in the muscular samples, but not in the vascular samples, whereas α-smooth muscle actin, a marker of smooth muscle cells, was detected only in the vascular samples. Moreover, SHR-SP had significantly greater BNP upregulation than WKY (P < 0.05) in the muscular samples. Conclusions. The LMD method enabled us to separately collect the muscular and vascular samples from myocardial sections and to selectively evaluate mRNA expressions of the individual tissue component.
Details
- Title: Subtitle
- Selective gene expression analysis of muscular and vascular components in hearts using laser microdissection method
- Creators
- Ayami Ikeda - Kurume UniversityHisashi Kai - Kurume UniversityHidemi Kajimoto - Kurume UniversitySuguru Yasuoka - Kurume UniversityMasayoshi Kage - Kurume University HospitalTsutomu Imaizumi - Kurume University
- Resource Type
- Journal article
- Publication Details
- International journal of vascular medicine, Vol.2012(2012), pp.420-424
- DOI
- 10.1155/2012/863410
- PMID
- 22778964
- PMCID
- PMC3384972
- NLM abbreviation
- Int J Vasc Med
- ISSN
- 2090-2824
- eISSN
- 2090-2832
- Grant note
- 22590565; 22791042; 24591104 / Japan Society for the Promotion of Science (http://data.elsevier.com/vocabulary/SciValFunders/501100001691)
- Language
- English
- Date published
- 01/01/2012
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics
- Record Identifier
- 9984961028702771
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