Journal article
Selectivity enhancement for glutamate with a Nafion/glutamate oxidase biosensor
Talanta (Oxford), Vol.43(7), pp.1157-1162
1996
DOI: 10.1016/0039-9140(95)01854-9
PMID: 18966594
Abstract
Response properties and selectivity are reported for glutamate biosensors constructed with a film of Nafion between the platinum anode and a layer of immobilized glutamate oxidase. The effects of enzyme loading, sample pH and temperature are established. Operation at pH 7.8 and 37 °C results in linearity up to 800 μM and a limit of detection of 0.3 μM. Nafion enhances selectivity for glutamate over test species that include ascorbic acid, uric acid and acetaminophen. Selectivity enhancement was greater over the anionic interferences because of electrostatic repulsion and the extent of this enhancement depends on the thickness of the Nafion layer. Even under ideal conditions, some interfering signal is observed when glutamate levels are ten-times less than ascorbate.
Details
- Title: Subtitle
- Selectivity enhancement for glutamate with a Nafion/glutamate oxidase biosensor
- Creators
- Shengtian PanMark A Arnold
- Resource Type
- Journal article
- Publication Details
- Talanta (Oxford), Vol.43(7), pp.1157-1162
- DOI
- 10.1016/0039-9140(95)01854-9
- PMID
- 18966594
- NLM abbreviation
- Talanta
- ISSN
- 0039-9140
- eISSN
- 1873-3573
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1996
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984216714102771
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