Journal article
Semen exosomes inhibit HIV infection and HIV-induced proinflammatory cytokine production independent of the activation state of primary lymphocytes
FEBS letters, Vol.594(4), pp.695-709
02/01/2020
DOI: 10.1002/1873-3468.13653
PMCID: PMC7042095
PMID: 31665815
Abstract
Semen exosomes (SE) inhibit HIV infection. However, the effect of SE on cell activation and inflammation remains unknown. We characterized the response of peripheral blood mononuclear cells (PBMCs) from HIV-uninfected and antiretroviral therapy-suppressed HIV-infected (HIV+) subjects to SE. Quiescent PBMCs or T-cell receptor (TCR)-activated PBMCs from HIV- and HIV+ donors were stimulated with SE in the presence/absence of ex vivo HIV infection. In HIV-infected PBMCs, SE did not reactivate HIV, did not induce lymphoblast development, nor increase CD69+/CD25+ numbers. Furthermore, SE inhibited de novo HIV infection without altering cell activation. SE also asynchronously downregulated HIV-inducible IL1 beta, IL8, and TNF alpha and upregulated CXCL10. These data suggest that SE inhibits HIV infection and production of HIV-induced proinflammatory cytokines while preserving lymphocyte activation.
Details
- Title: Subtitle
- Semen exosomes inhibit HIV infection and HIV-induced proinflammatory cytokine production independent of the activation state of primary lymphocytes
- Creators
- Jennifer L. Welch - Veterans Health AdministrationThomas M. Kaufman - Veterans Health AdministrationJack T. Stapleton - Veterans Health AdministrationChioma M. Okeoma - Stony Brook University
- Resource Type
- Journal article
- Publication Details
- FEBS letters, Vol.594(4), pp.695-709
- DOI
- 10.1002/1873-3468.13653
- PMID
- 31665815
- PMCID
- PMC7042095
- NLM abbreviation
- FEBS Lett
- ISSN
- 0014-5793
- eISSN
- 1873-3468
- Publisher
- Wiley
- Number of pages
- 15
- Grant note
- BX000207; NIH 5T32AI343 / National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA VA Merit Review; US Department of Veterans Affairs 5T32AI007533-18 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA DA042348 / National Institute on Drug Abuse (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA)
- Language
- English
- Date published
- 02/01/2020
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984297317102771
Metrics
8 Record Views