Journal article
Sensitive Drug-Resistance Assays Reveal Long-Term Persistence of HIV-1 Variants with the K103N Nevirapine (NVP) Resistance Mutation in Some Women and Infants after the Administration of Single-Dose NVP: HIVNET 012
The Journal of infectious diseases, Vol.192(1), pp.24-29
07/01/2005
DOI: 10.1086/430742
PMID: 15942890
Abstract
BackgroundThe HIV Network for Prevention Trials (HIVNET) 012 trial showed that NVP resistance (NVPR) emerged in some women and children after the administration of single-dose nevirapine (SD-NVP). We tested whether K103N-containing human immunodeficiency virus (HIV)-1 variants persisted in women and infants 1 year or more after the administration of SD-NVP
MethodsWe analyzed samples from 9 women and 5 infants in HIVNET 012 who had NVPR 6-8 weeks after the administration of SD-NVP. Samples were analyzed with the ViroSeq system and with 2 sensitive resistance assays, LigAmp and TyHRT
ResultsViroSeq detected the K103N mutation in 8 of 9 women and in 2 of 5 infants. LigAmp detected the K103N mutation at low levels in 8 of 9 women and in 4 of 5 infants. K103N was not detected by ViroSeq 12-24 months after the administration of SD-NVP but was detected by LigAmp in 3 of 9 women and in 1 of 5 infants. K103N was also detected in those samples by use of the TyHRT assay
ConclusionsK103N-containing variants persist in some women and infants for 1 year or more after the administration of SD-NVP. Sensitive resistance assays may provide new insight into the impact of antiretroviral drug exposure on HIV-1 evolution
Details
- Title: Subtitle
- Sensitive Drug-Resistance Assays Reveal Long-Term Persistence of HIV-1 Variants with the K103N Nevirapine (NVP) Resistance Mutation in Some Women and Infants after the Administration of Single-Dose NVP: HIVNET 012
- Creators
- Tamara Flys - Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, andDwight V Nissley - Gene Regulation and Chromosome Biology Laboratory, Reverse Transcription and Molecular Biology Section, Basic Research Program, Science Applications International Corporation-FrederickCassidy W Claasen - National Cancer Institute-Frederick, Frederick, Maryland; Departments ofDana Jones - Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, andChanjuan Shi - Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, andLaura A Guay - Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, andPhilippa Musoke - Paediatrics andFrancis Mmiro - Obstetrics and Gynaecology, Makerere University, Kampala, UgandaJeffrey N Strathern - National Cancer Institute-Frederick, Frederick, Maryland; Departments ofJ. Brooks Jackson - Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, andJames R Eshleman - Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, andSusan H Eshleman - Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, and
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.192(1), pp.24-29
- Publisher
- The University of Chicago Press
- DOI
- 10.1086/430742
- PMID
- 15942890
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 07/01/2005
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984047625202771
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