Sensitivity to radiation of human normal, hyperthyroid, and neoplastic thyroid epithelial cells in primary culture

R C Miller; T Hiraoka; K J Kopecky; N Nakamura; M P Jones; T Ito; K H Clifton

doi:10.2307/3577023

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Sensitivity to radiation of human normal, hyperthyroid, and neoplastic thyroid epithelial cells in primary culture

Journal article

Peer reviewed

Sensitivity to radiation of human normal, hyperthyroid, and neoplastic thyroid epithelial cells in primary culture

R C Miller, T Hiraoka, K J Kopecky, N Nakamura, M P Jones, T Ito and K H Clifton

Radiation research, Vol.111(1), pp.81-91

07/1987

DOI: 10.2307/3577023

PMID: 3602357

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Abstract

Samples of thyroid tissue removed surgically from 63 patients were cultured in vitro and exposed to X irradiation to investigate the radiosensitivities of various types of thyroid epithelial cells. A total of 76 samples were obtained, including neoplastic cells from patients with papillary carcinoma (PC) or follicular adenoma (FA), cells from hyperthyroidism (HY) patients, and normal cells from the surgical margins of PC and FA patients. Culturing of the cells was performed in a manner which has been shown to yield a predominance of epithelial cells. Results of colony formation assays indicated that cells from HY and FA patients were the least radiosensitive: when adjusted to the overall geometric mean plating efficiency of 5.5%, the average mean lethal dose Do was 97.6 cGy for HY cells and 96.7 and 94.3 cGy, respectively for neoplastic and normal cells from FA patients. Cells from PC patients were more radiosensitive, normal cells having an adjusted average Do of 85.0 cGy and PC cells a significantly (P = 0.05) lower average Do of 74.4 cGy. After allowing for this variation by cell type, in vitro radiosensitivity was not significantly related to age at surgery (P = 0.82) or sex (P = 0.10). These results suggest that malignant thyroid cells may be especially radiosensitive.

Cells, Cultured - radiation effects

Thyroid Gland - radiation effects

Humans

Hyperthyroidism - pathology

Radiation Tolerance

Adenoma - pathology

Carcinoma, Papillary - pathology

Cell Survival - radiation effects

Dose-Response Relationship, Radiation

Epithelium - radiation effects

Thyroid Neoplasms - pathology

Details

Title: Subtitle: Sensitivity to radiation of human normal, hyperthyroid, and neoplastic thyroid epithelial cells in primary culture
Creators: R C Miller
T Hiraoka
K J Kopecky
N Nakamura
M P Jones
T Ito
K H Clifton
Resource Type: Journal article
Publication Details: Radiation research, Vol.111(1), pp.81-91
Publisher: United States
DOI: 10.2307/3577023
PMID: 3602357
ISSN: 0033-7587
eISSN: 1938-5404
Language: English
Date published: 07/1987
Academic Unit: Statistics and Actuarial Science; Biostatistics; Public Policy Center (Archive)
Record Identifier: 9983985812902771

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