Journal article
Sensitivity to theta-burst timing permits LTP in dorsal striatal adult brain slice
Journal of neurophysiology, Vol.110(9), pp.2027-2036
11/01/2013
DOI: 10.1152/jn.00115.2013
PMCID: PMC4073966
PMID: 23926032
Abstract
Long-term potentiation (LTP) of excitatory afferents to the dorsal striatum likely occurs with learning to encode new skills and habits, yet corticostriatal LTP is challenging to evoke reliably in brain slice under physiological conditions. Here we test the hypothesis that stimulating striatal afferents with theta-burst timing, similar to recently reported in vivo temporal patterns corresponding to learning, evokes LTP. Recording from adult mouse brain slice extracellularly in 1 mM Mg2+, we find LTP in dorsomedial and dorsolateral striatum is preferentially evoked by certain theta-burst patterns. In particular, we demonstrate that greater LTP is produced using moderate intraburst and high theta-range frequencies, and that pauses separating bursts of stimuli are critical for LTP induction. By altering temporal pattern alone, we illustrate the importance of burst-patterning for LTP induction and demonstrate that corticostriatal long-term depression is evoked in the same preparation. In accord with prior studies, LTP is greatest in dorsomedial striatum and relies on N-methyl-D-aspartate receptors. We also demonstrate a requirement for both G(q)- and G(s/olf)-coupled pathways, as well as several kinases associated with memory storage: PKC, PKA, and ERK. Our data build on previous reports of activity-directed plasticity by identifying effective values for distinct temporal parameters in variants of theta-burst LTP induction paradigms. We conclude that those variants which best match reports of striatal activity during learning behavior are most successful in evoking dorsal striatal LTP in adult brain slice without altering artificial cerebrospinal fluid. Future application of this approach will enable diverse investigations of plasticity serving striatal-based learning.
Details
- Title: Subtitle
- Sensitivity to theta-burst timing permits LTP in dorsal striatal adult brain slice
- Creators
- Sarah L. Hawes - George Mason UniversityFawad Gillani - George Mason UniversityRebekah C. Evans - George Mason UniversityElizabeth A. Benkert - George Mason UniversityKim T. Blackwell - George Mason University
- Resource Type
- Journal article
- Publication Details
- Journal of neurophysiology, Vol.110(9), pp.2027-2036
- DOI
- 10.1152/jn.00115.2013
- PMID
- 23926032
- PMCID
- PMC4073966
- NLM abbreviation
- J Neurophysiol
- ISSN
- 0022-3077
- eISSN
- 1522-1598
- Publisher
- Amer Physiological Soc
- Number of pages
- 10
- Grant note
- R01AA016022 / NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Alcohol Abuse & Alcoholism (NIAAA) N00014-10-1-0198; NIAAA R01 / Office of Naval Research Multidisciplinary University Research Initiative Grant
- Language
- English
- Date published
- 11/01/2013
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute
- Record Identifier
- 9984446553102771
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