Journal article
Sensitization of Non-permissive Laboratory Mice to SARS-CoV-2 with a Replication-Deficient Adenovirus Expressing Human ACE2
STAR Protocols, Vol.1(3), pp.100169-100169
12/18/2020
DOI: 10.1016/j.xpro.2020.100169
PMID: 33377063
Abstract
Common laboratory mice such as BALB/c and C57BL/6 mice are not permissive to SARS-CoV2 infection. Sensitization of laboratory mice with Adenovirus expressing human ACE2 (Ad5-hACE2) provides a rapid model for testing viral intervention in vivo. Despite the lack of lethal outcome, Ad5-hACE2-sensitized mice show 20% weight loss on average upon viral challenge with infectious virus being detected at the site of sensitization. This protocol describes the sensitization and subsequent infection of common laboratory mice for use in testing anti-viral interventions.
For complete details on the use and execution of this protocol, please refer to Sun et al. (2020).
[Display omitted]
•Sensitization of non-permissive laboratory mice to SARS-CoV-2 infection by Ad5-hACE2•A mouse model for SARS-CoV-2 infection for testing anti-viral interventions•Rapid model applicable to mice with different genetic backgrounds
Common laboratory mice such as BALB/c and C57BL/6 mice are not permissive to SARS-CoV-2 infection. Sensitization of laboratory mice with Adenovirus expressing human ACE2 (Ad5-hACE2) provides a rapid model for testing viral intervention in vivo. Despite the lack of lethal outcome, Ad5-hACE2-sensitized mice show 20% weight loss on average upon viral challenge with infectious virus being detected at the site of sensitization. This protocol describes the sensitization and subsequent infection of common laboratory mice for use in testing anti-viral interventions.
Details
- Title: Subtitle
- Sensitization of Non-permissive Laboratory Mice to SARS-CoV-2 with a Replication-Deficient Adenovirus Expressing Human ACE2
- Creators
- Lok-Yin Roy Wong - Department of Microbiology and Immunology, University of Iowa, Iowa City, IA 52242, USAKun Li - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USAJing Sun - State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510182, ChinaZhen Zhuang - State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510182, ChinaJincun Zhao - State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510182, ChinaPaul B McCray - Department of Microbiology and Immunology, University of Iowa, Iowa City, IA 52242, USAStanley Perlman - Department of Microbiology and Immunology, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- STAR Protocols, Vol.1(3), pp.100169-100169
- DOI
- 10.1016/j.xpro.2020.100169
- PMID
- 33377063
- NLM abbreviation
- STAR Protoc
- ISSN
- 2666-1667
- eISSN
- 2666-1667
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100007197, name: U.S. Public Health Service
- Language
- English
- Date published
- 12/18/2020
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics); Internal Medicine
- Record Identifier
- 9984070874602771
Metrics
16 Record Views