Sentinel lymph node mapping of invasive urinary bladder cancer in animal models using invisible light

Deborah W Knapp; Larry G Adams; Alec M Degrand; Jacqueline D Niles; José A Ramos-Vara; Ann B Weil; Michael A O'Donnell; Michael D Lucroy; John V Frangioni

doi:10.1016/j.eururo.2007.07.007

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Sentinel lymph node mapping of invasive urinary bladder cancer in animal models using invisible light

Journal article

Peer reviewed

Sentinel lymph node mapping of invasive urinary bladder cancer in animal models using invisible light

Deborah W Knapp, Larry G Adams, Alec M Degrand, Jacqueline D Niles, José A Ramos-Vara, Ann B Weil, Michael A O'Donnell, Michael D Lucroy and John V Frangioni

European urology, Vol.52(6), pp.1700-1709

12/2007

DOI: 10.1016/j.eururo.2007.07.007

PMCID: PMC2385787

PMID: 17646044

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http://doi.org/10.1016/j.eururo.2007.07.007View

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Abstract

With conventional methodology, sentinel lymph node (SLN) mapping of invasive urinary bladder cancer is technically challenging. This study was performed to determine the utility of invisible, near-infrared fluorescent (NIRF) light for patient-specific SLN mapping, in real time under complete image guidance. Lymphatic tracers, injection volume, NIRF excitation fluence rate, light collection of emitted fluorescence, and degree of bladder distension were systematically optimized in normal dogs and pigs. SLN mapping was then performed in pet dogs with naturally occurring invasive transitional cell carcinoma (InvTCC) of the urinary bladder, which closely mimics the human disease. NIRF albumin (hydrodynamic diameter [HD], 7.4 nm) and NIRF quantum dots (15-20 nm HD) injected into the bladder wall resulted in identification of draining lymph nodes (LNs) in under 3 min. In both species, considerable variability in the lymphatic drainage was observed among individuals. Optimal SLN mapping was achieved with the use of superficial, serosal injection of NIRF tracer, with the bladder distended to an intraluminal pressure of 20-40 cm H(2)O. In dogs with InvTCC, NIRF tracers identified SLNs that were confirmed histologically to harbor metastases. The use of invisible NIRF light permits real-time, patient-specific identification of SLNs that drain bladder cancer. Intraluminal bladder pressure is a key parameter that needs to be controlled for optimal results.

Animals

Carcinoma, Transitional Cell - pathology

Neoplasm Invasiveness

Swine

Dogs

Urinary Bladder Neoplasms - pathology

Female

Male

Sentinel Lymph Node Biopsy

Disease Models, Animal

Details

Title: Subtitle: Sentinel lymph node mapping of invasive urinary bladder cancer in animal models using invisible light
Creators: Deborah W Knapp - Department of Veterinary Clinical Sciences, and Purdue Cancer Center and Oncological Sciences Center, West Lafayette, IN 47907, USA. knappd@purdue.edu
Larry G Adams
Alec M Degrand
Jacqueline D Niles
José A Ramos-Vara
Ann B Weil
Michael A O'Donnell
Michael D Lucroy
John V Frangioni
Resource Type: Journal article
Publication Details: European urology, Vol.52(6), pp.1700-1709
DOI: 10.1016/j.eururo.2007.07.007
PMID: 17646044
PMCID: PMC2385787
NLM abbreviation: Eur Urol
ISSN: 0302-2838
eISSN: 1873-7560
Publisher: Switzerland
Grant note: R21 CA110185 / NCI NIH HHS R21-CA-110185 / NCI NIH HHS R21 CA110185-01 / NCI NIH HHS R01 CA115296-02 / NCI NIH HHS R01 CA115296-01A1 / NCI NIH HHS R01 CA115296 / NCI NIH HHS R01-CA-115296 / NCI NIH HHS
Language: English
Date published: 12/2007
Academic Unit: Urology
Record Identifier: 9984051758702771

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