Journal article
Separation of hydroxyl protected heparin derived disaccharides using reversed-phase high-performance liquid chromatography
Journal of chromatography. A, Vol.705(2), pp.369-373
06/30/1995
DOI: 10.1016/0021-9673(95)00293-V
PMID: 7640772
Abstract
A simple and efficient method for the separation of hydrophobic derivatives of glycosaminoglycan-derived disaccharides is described. Hydroxyl-protected derivatives of a trisulfated disaccharide, prepared from heparin using heparin lyase, were separated by reversed-phase high-performance liquid chromatography. These disaccharide derivatives differed by the number, position, and stereochemistry of acetyl and pivaloyl groups. Separation was achieved on a C18 column using a reversed gradient of ammonium sulfate in water. This method has application in the purification of disaccharide derivatives being used as chiral synthons in the preparation of higher oligosaccharides.
Details
- Title: Subtitle
- Separation of hydroxyl protected heparin derived disaccharides using reversed-phase high-performance liquid chromatography
- Creators
- R J Kerns - University of IowaR J Linhardt - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of chromatography. A, Vol.705(2), pp.369-373
- DOI
- 10.1016/0021-9673(95)00293-V
- PMID
- 7640772
- ISSN
- 0021-9673
- Grant note
- GM08365 / NIGMS NIH HHS
- Language
- English
- Date published
- 06/30/1995
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984366028402771
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