Journal article
Separation of leukemic cells into proliferative and quiescent subpopulations by centrifugal elutriation
Cancer research (Chicago, Ill.), Vol.37(11), pp.3876-3880
11/01/1977
PMID: 71201
Abstract
Centrifugal elutriation was used to separate human acute leukemia cells into proliferative and quiescent subpopulations. Ten bone marrow specimens and 5 peripheral blood specimens were subjected to centrifugal elutriations. From each patient, leukemic cell subpopulations were obtained for which the [3H]thymidine labeling index differed by 10- to 30-fold. In 6 of the marrow specimens and in 2 of the peripheral blood specimens, cell subpopulations were obtained for which the labeling index exceeded 20%. In 5 marrow specimens, subpopulations were obtained for which the labeling index exceeded 40%. Preliminary studies of the uptake of 1-beta-D-arabinofuranosylcytosine and 5-azacytidine failed to show any correlation between drug uptake and the proliferative characteristics of the leukemic subpopulations.
Details
- Title: Subtitle
- Separation of leukemic cells into proliferative and quiescent subpopulations by centrifugal elutriation
- Creators
- H D PreislerI WalczakJ RenickY M Rustum
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.37(11), pp.3876-3880
- PMID
- 71201
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 11/01/1977
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359816602771
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