Journal article
Separation of metalloporphyrins from metallation reactions by liquid chromatography and electrophoresis
Journal of chromatography. Biomedical applications, Vol.416(C), pp.71-80
1987
DOI: 10.1016/0378-4347(87)80486-3
PMID: 3597643
Abstract
The analytical separation of the indium and manganese complexes of three synthetic, meso-substituted, water-soluble porphyrins from their respective free bases in metallation reaction mixtures is described. The ligands tetra-3N-methylpyridyl porphyrin, tetra-4N-methylpyridyl porphyrin and tetra-N,N,N-trimethylanilinium porphyrin are complexed with In (III) and Mn (III) and are separated from residual free base by high-performance liquid chromatography (HPLC) in acidic conditions with gradient elution on ODS bonded stationary phase. Electrophoretic separation is achieved on both cellulose polyacetate strips and polyacrylamide tube gels under basic conditions. Although analytical separations can be achieved by both HPLC and electrophoresis, only HPLC is suitable for the development of preparative scale separations. Column chromatography, ion-pairing and ion-suppression HPLC techniques fail to separate such highly charged and related aromatic compounds.
Details
- Title: Subtitle
- Separation of metalloporphyrins from metallation reactions by liquid chromatography and electrophoresis
- Creators
- G.Andrew Duff - Department of Chemistry and Center for Health Sciences, Lehigh University, Seeley G. Mudd No. 6, Bethlehem, PA 18015 U.S.ASandra A. Yeager - Department of Chemistry, Millersville University, Millersville, PA 17551 U.S.AArun K. Singhal - University of Iowa, Cardiothoracic SurgeryBeverly C. Pestel - Department of Chemistry, University of Wisconsin-Eau Claire, Eau Claire, WI 54701 U.S.AJoel M. Ressner - Department of Chemistry, West Chester University, West Chester, PA 19380 U.S.ANatalie Foster - Department of Chemistry and Center for Health Sciences, Lehigh University, Seeley G. Mudd No. 6, Bethlehem, PA 18015 U.S.A
- Resource Type
- Journal article
- Publication Details
- Journal of chromatography. Biomedical applications, Vol.416(C), pp.71-80
- DOI
- 10.1016/0378-4347(87)80486-3
- PMID
- 3597643
- NLM abbreviation
- J Chromatogr B Biomed Appl
- ISSN
- 0378-4347
- Publisher
- Elsevier B.V
- Number of pages
- 10
- Language
- English
- Date published
- 1987
- Academic Unit
- Surgery; Cardiothoracic Surgery
- Record Identifier
- 9984051591002771
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