Journal article
Septal Localization of FtsQ, an Essential Cell Division Protein in Escherichia coli
Journal of bacteriology, Vol.181(2), pp.521-530
01/1999
DOI: 10.1128/jb.181.2.521-530.1999
PMCID: PMC93406
PMID: 9882666
Abstract
Septation in
Escherichia coli
requires several gene products. One of these, FtsQ, is a simple bitopic membrane protein with a short cytoplasmic N terminus, a membrane-spanning segment, and a periplasmic domain. We have constructed a merodiploid strain that expresses both FtsQ and the fusion protein green fluorescent protein (GFP)-FtsQ from single-copy chromosomal genes. The
gfp-ftsQ
gene complements a null mutation in
ftsQ
. Fluorescence microscopy revealed that GFP-FtsQ localizes to the division site. Replacing the cytoplasmic and transmembrane domains of FtsQ with alternative membrane anchors did not prevent the localization of the GFP fusion protein, while replacing the periplasmic domain did, suggesting that the periplasmic domain is necessary and sufficient for septal targeting. GFP-FtsQ localization to the septum depended on the cell division proteins FtsZ and FtsA, which are cytoplasmic, but not on FtsL and FtsI, which are bitopic membrane proteins with comparatively large periplasmic domains. In addition, the septal localization of ZipA apparently did not require functional FtsQ. Our results indicate that FtsQ is an intermediate recruit to the division site.
Details
- Title: Subtitle
- Septal Localization of FtsQ, an Essential Cell Division Protein in Escherichia coli
- Creators
- Joseph C Chen - Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115David S Weiss - Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115Jean-Marc Ghigo - Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115Jon Beckwith - Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.181(2), pp.521-530
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/jb.181.2.521-530.1999
- PMID
- 9882666
- PMCID
- PMC93406
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Language
- English
- Date published
- 01/1999
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984002382102771
Metrics
13 Record Views