Sequence similarity of calreticulin with a Ca2(+)-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells

K H Krause; H K Simmerman; L R Jones; K P Campbell

doi:10.1042/bj2700545

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Sequence similarity of calreticulin with a Ca2(+)-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells

Journal article

Open access

Peer reviewed

Sequence similarity of calreticulin with a Ca2(+)-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells

K H Krause, H K Simmerman, L R Jones and K P Campbell

The Biochemical journal, Vol.270(2), pp.545-548

09/01/1990

DOI: 10.1042/bj2700545

PMCID: PMC1131758

PMID: 2400400

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Abstract

HL-60 cells possess a 60 kDa Ca2(+)-binding protein that is contained in a discrete subcellular compartment, referred to as calciosomes. Subcellular fractionation studies have suggested that, in HL-60 cells, this intracellular compartment is an Ins(1,4,5)P3-sensitive Ca2+ store. In order to investigate the structural relationship of the 60 kDa Ca2(+)-binding protein of HL-60 cells to other Ca2(+)-binding proteins, we have purified the protein by ammonium sulphate extraction, acid precipitation, and DEAE-cellulose and phenyl-Sepharose column chromatography. The N-terminal sequence of the protein shows 93% identity with rabbit muscle calreticulin, a recently cloned sarcoplasmic reticulum Ca2(+)-binding protein. No amino acid sequence similarity with calsequestrin was found, although the purified protein cross-reacted with anti-calsequestrin antibodies. The calreticulin-related protein of HL-60 cells might play a role as an intravesicular Ca2(+)-binding protein of an Ins(1,4,5)P3-sensitive Ca2+ store.

Chromatography

Calsequestrin

Amino Acid Sequence

Rabbits

Cell Fractionation

Molecular Weight

Calcium - metabolism

Humans

Molecular Sequence Data

Immunoblotting

Inositol 1,4,5-Trisphosphate - pharmacology

Organelles - analysis

Myocardium - analysis

Sequence Homology, Nucleic Acid

Calreticulin

Animals

Leukemia, Promyelocytic, Acute

Calcium-Binding Proteins - isolation & purification

Tumor Cells, Cultured

Muscles - analysis

Details

Title: Subtitle: Sequence similarity of calreticulin with a Ca2(+)-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells
Creators: K H Krause - Division of Infectious Diseases, Geneva University Hospital, Switzerland
H K Simmerman
L R Jones
K P Campbell
Resource Type: Journal article
Publication Details: The Biochemical journal, Vol.270(2), pp.545-548
DOI: 10.1042/bj2700545
PMID: 2400400
PMCID: PMC1131758
NLM abbreviation: Biochem J
ISSN: 0264-6021
eISSN: 1470-8728
Publisher: England
Grant note: HL-37187 / NHLBI NIH HHS
Language: English
Date published: 09/01/1990
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984068379302771

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