Journal article
Sequential Appearance of Inflammatory Mediators in Rat Bronchoalveolar Lavage Fluid After Oleic Acid-Induced Lung Injury
Experimental lung research, Vol.22(1), pp.33-49
1996
DOI: 10.3109/01902149609074016
PMID: 8838134
Abstract
The oleic acid (OA) model of rat lung injury was originally developed as a model of fat embolism syndrome. A single intravenous dose of pure OA causes an acute diffuse lung injury, which, in its initial stages, histologically and physiologically resembles human ARDS. Rat lungs acutely injured by intravenous OA manifest increased levels of ICAM-1 within 30-60 min. This study shows that human umbilical vein endothelial cells (HUVEC) can be used in a bioassay to reveal some of the adhesion molecule stimulating activities present in bronchoalveolar lavage fluid (BALF) from post-OA-injected rats: (1) There is an as yet unidentified ICAM-1 and ELAM (E Selectin) inducing activity in BALF within 15 min of OA injection that is not TNFα; there is very little measurable tumor necrosis factor-alpha (TNFα) in 15 min BALF (BALF15). (2) BALF15 also stimulates cultured macrophage derived from BALF of normal rat lungs to produce TNFα. (3) By 60 min after OA injection, 50-75% of the ICAM-1 and ELAM inducing activity in BALF (BALF60) is TNFα; BALF60 contains about 250-280 pg/mL TNFα. The other 25-50% adhesion molecule-inducing activity in BALF60 is unidentified. (4) The ICAM-1-inducing activity of pure TNFα was equal to that of BALF60 containing equivalent concentrations of TNFα. The ELAM inducing activity of pure TNFα, however, was about 1/2 that of BALF60 containing equivalent concentrations of TNFα. The time courses for ICAM and ELAM stimulation with pure TNFα or BALF60 containing equivalent levels of TNFα were the same. The identity of the mediators in BALF15 and BALF60 that are not TNFα and the mechanisms by which OA injection stimulates cytokine production remain to be elucidated.
Details
- Title: Subtitle
- Sequential Appearance of Inflammatory Mediators in Rat Bronchoalveolar Lavage Fluid After Oleic Acid-Induced Lung Injury
- Creators
- Sergei Syrbu - 1Departments of Pharmacology, Surgery, and Medicine, University of Connecticut Health Center, Farmington, Connecticut, USARoger S Thrall - 1Departments of Pharmacology, Surgery, and Medicine, University of Connecticut Health Center, Farmington, Connecticut, USAHenry M Smilowitz - 1Departments of Pharmacology, Surgery, and Medicine, University of Connecticut Health Center, Farmington, Connecticut, USA
- Resource Type
- Journal article
- Publication Details
- Experimental lung research, Vol.22(1), pp.33-49
- Publisher
- Informa UK Ltd
- DOI
- 10.3109/01902149609074016
- PMID
- 8838134
- ISSN
- 0190-2148
- eISSN
- 1521-0499
- Language
- English
- Date published
- 1996
- Academic Unit
- Pathology
- Record Identifier
- 9984047722302771
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