Sequential activation of CD8+ T cells in the draining lymph nodes in response to pulmonary virus infection

Heesik Yoon; Kevin L Legge; Sun-sang J Sung; Thomas J Braciale

doi:10.4049/jimmunol.179.1.391

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Sequential activation of CD8+ T cells in the draining lymph nodes in response to pulmonary virus infection

Journal article

Open access

Peer reviewed

Sequential activation of CD8+ T cells in the draining lymph nodes in response to pulmonary virus infection

Heesik Yoon, Kevin L Legge, Sun-sang J Sung and Thomas J Braciale

The Journal of immunology (1950), Vol.179(1), pp.391-399

07/01/2007

DOI: 10.4049/jimmunol.179.1.391

PMID: 17579060

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Abstract

We have used a TCR-transgenic CD8+ T cell adoptive transfer model to examine the tempo of T cell activation and proliferation in the draining lymph nodes (DLN) in response to respiratory virus infection. The T cell response in the DLN differed for mice infected with different type A influenza strains with the onset of T cell activation/proliferation to the A/JAPAN virus infection preceding the A/PR8 response by 12-24 h. This difference in T cell activation/proliferation correlated with the tempo of accelerated respiratory DC (RDC) migration from the infected lungs to the DLN in response to influenza virus infection, with the migrant RDC responding to the A/JAPAN infection exhibiting a more rapid accumulation in the lymph nodes (i.e., peak migration for A/JAPAN at 18 h, A/PR8 at 24-36 h). Furthermore, in vivo administration of blocking anti-CD62L Ab at various time points before/after infection revealed that the virus-specific CD8+ T cells entered the DLN and activated in a sequential "conveyor belt"-like fashion. These results indicate that the tempo of CD8+ T cell activation/proliferation after viral infection is dependent on the tempo of RDC migration to the DLN and that T cell activation occurs in an ordered sequential fashion.

Influenza A Virus, H1N1 Subtype - immunology

Lymph Nodes - pathology

CD8-Positive T-Lymphocytes - transplantation

Dendritic Cells - immunology

Dendritic Cells - pathology

Influenza A Virus, H2N2 Subtype - immunology

Adoptive Transfer

Cell Movement - genetics

Cell Movement - immunology

Lung Diseases - virology

Lymphocyte Activation - genetics

Lymphocyte Activation - immunology

Dendritic Cells - virology

Orthomyxoviridae Infections - pathology

Cells, Cultured

Lymph Nodes - metabolism

Mice, Transgenic

Virus Replication - immunology

Lymph Nodes - immunology

Lung Diseases - immunology

Animals

CD8-Positive T-Lymphocytes - virology

Mice

Mice, Inbred BALB C

Kinetics

Lung Diseases - pathology

CD8-Positive T-Lymphocytes - immunology

Orthomyxoviridae Infections - immunology

Details

Title: Subtitle: Sequential activation of CD8+ T cells in the draining lymph nodes in response to pulmonary virus infection
Creators: Heesik Yoon - Carter Immunology Center, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
Kevin L Legge
Sun-sang J Sung
Thomas J Braciale
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.179(1), pp.391-399
Publisher: United States
DOI: 10.4049/jimmunol.179.1.391
PMID: 17579060
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AI 15608 / NIAID NIH HHS HL 70065 / NHLBI NIH HHS HL 33391 / NHLBI NIH HHS AI 37293 / NIAID NIH HHS
Language: English
Date published: 07/01/2007
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984046915502771

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