Journal article
Serine-selective membrane probe based on immobilized anaerobic bacteria and a potentiometric ammonia gas sensor
Analytica chimica acta, Vol.128(C), pp.121-127
1981
DOI: 10.1016/S0003-2670(01)84089-7
Abstract
A new class of bioselective membrane probes using anaerobic bacteria is introduced with the successful construction of a L-serine-selective probe consisting of
Clostridium acidiurici cells coupled to a potentiometric ammonia gas sensor. The intact cells containing the enzyme serine dehydratase are physically immobilized at the electrode surface in conjunction with iron(II) stearate, which is shown to enhance response characteristics. The potential vs. log concentration plot is linear from 1.6 × 10
-2 to 1.8 × 10
-4
M serine with an average slope of 54 mV/decade and response times of 3–5 min. Optimal behavior of the probe is retained even in non-deaerated media for at least three days, and significant interference is posed only by L-glutamine. Quantitative conversion of serine is demonstrated over the linear concentration range, suggesting possible analytical or clinical applications for these probes utilizing anaerobic bacteria
Details
- Title: Subtitle
- Serine-selective membrane probe based on immobilized anaerobic bacteria and a potentiometric ammonia gas sensor
- Creators
- C.L Di PaolantonioM.A ArnoldG.A Rechnttz
- Resource Type
- Journal article
- Publication Details
- Analytica chimica acta, Vol.128(C), pp.121-127
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0003-2670(01)84089-7
- ISSN
- 0003-2670
- eISSN
- 1873-4324
- Language
- English
- Date published
- 1981
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Center for Biocatalysis and Bioprocessing; Chemistry
- Record Identifier
- 9984216571302771
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