Seroepidemiology of Middle East respiratory syndrome (MERS) coronavirus in Saudi Arabia (1993) and Australia (2014) and characterisation of assay specificity

M G Hemida; R A Perera; R A Al Jassim; G Kayali; L Y Siu; P Wang; K W Chu; S Perlman; M A Ali; A Alnaeem; Y Guan; L L Poon; L Saif; M Peiris

doi:10.2807/1560-7917.ES2014.19.23.20828

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Seroepidemiology of Middle East respiratory syndrome (MERS) coronavirus in Saudi Arabia (1993) and Australia (2014) and characterisation of assay specificity

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Seroepidemiology of Middle East respiratory syndrome (MERS) coronavirus in Saudi Arabia (1993) and Australia (2014) and characterisation of assay specificity

M G Hemida, R A Perera, R A Al Jassim, G Kayali, L Y Siu, P Wang, K W Chu, S Perlman, M A Ali, A Alnaeem, …

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, Vol.19(23), pp.2-7

06/12/2014

DOI: 10.2807/1560-7917.ES2014.19.23.20828

PMCID: PMC4674219

PMID: 24957744

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Abstract

The pseudoparticle virus neutralisation test (ppNT) and a conventional microneutralisation (MN) assay are specific for detecting antibodies to Middle East respiratory syndrome coronavirus (MERS-CoV) when used in seroepidemiological studies in animals. Genetically diverse MERS-CoV appear antigenically similar in MN tests. We confirm that MERS-CoV was circulating in dromedaries in Saudi Arabia in 1993. Preliminary data suggest that feral Australian dromedaries may be free of MERS-CoV but larger confirmatory studies are needed.

Cattle - blood

Prevalence

Immunoglobulin G - blood

Humans

Seroepidemiologic Studies

Camelus - blood

Antibodies, Viral - blood

Australia - epidemiology

Coronavirus - immunology

Animals

Coronavirus Infections - epidemiology

Coronavirus Infections - virology

Coronavirus Infections - blood

Respiratory Tract Infections - epidemiology

Respiratory Tract Infections - virology

Neutralization Tests - methods

Saudi Arabia - epidemiology

Antibodies, Neutralizing - blood

Respiratory Tract Infections - blood

Coronavirus - isolation & purification

Details

Title: Subtitle: Seroepidemiology of Middle East respiratory syndrome (MERS) coronavirus in Saudi Arabia (1993) and Australia (2014) and characterisation of assay specificity
Creators: M G Hemida - These authors contributed equally to this work
R A Perera
R A Al Jassim
G Kayali
L Y Siu
P Wang
K W Chu
S Perlman
M A Ali
A Alnaeem
Y Guan
L L Poon
L Saif
M Peiris
Resource Type: Journal article
Publication Details: Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, Vol.19(23), pp.2-7
DOI: 10.2807/1560-7917.ES2014.19.23.20828
PMID: 24957744
PMCID: PMC4674219
NLM abbreviation: Euro Surveill
ISSN: 1025-496X
eISSN: 1560-7917
Publisher: Sweden
Grant note: R01 AI091322 / NIAID NIH HHS P01 AI060699 / NIAID NIH HHS HHSN266200700005C / PHS HHS HHSN266200700005C / NIAID NIH HHS
Language: English
Date published: 06/12/2014
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777476602771

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