Serotonergic mechanisms are necessary for central respiratory chemoresponsiveness in situ

Andrea E Corcoran; George B Richerson; Michael B Harris

doi:10.1016/j.resp.2013.02.015

Back

Serotonergic mechanisms are necessary for central respiratory chemoresponsiveness in situ

Journal article

Peer reviewed

Serotonergic mechanisms are necessary for central respiratory chemoresponsiveness in situ

Andrea E Corcoran, George B Richerson and Michael B Harris

Respiratory physiology & neurobiology, Vol.186(2), pp.214-220

04/01/2013

DOI: 10.1016/j.resp.2013.02.015

PMCID: PMC3645885

PMID: 23454177

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/3645885View

Open Access

Abstract

Evidence from in vivo and in vitro experiments conclude that serotonin (5-HT) neurons are involved in and play an important role in central respiratory CO2/H(+) chemosensitivity. This study was designed to assess the importance of 5-HT neurons and 5-HT receptor activation in the frequency and amplitude components of the hypercapnic response of the respiratory network in the unanesthetized perfused in situ juvenile rat brainstem preparation that exhibits patterns of phrenic nerve discharge similar to breathing in vivo. Exposure to a hypercapnic perfusate increased phrenic burst frequency and/or amplitude, the neural correlates of breathing frequency and tidal volume in vivo. Hypercapnic responses were also assessed during exposure to ketanserin (5-HT2 receptor antagonist), and 8-OH-DPAT (inhibiting 5-HT neurons via 5-HT1A autoreceptors). Neither of these drugs substantially altered baseline activity, however, both abolished hypercapnic responses of the respiratory network. These data illustrate that 5-HT neurons and 5-HT receptor activation are not required for respiratory rhythm generation per se, but are critical for CO2 responses in situ, supporting the hypothesis that 5-HT neurons play an important role in central ventilatory chemosensitivity in vivo.

Phrenic Nerve - metabolism

Ketanserin - pharmacology

Brain Stem - metabolism

Rats

8-Hydroxy-2-(di-n-propylamino)tetralin - pharmacology

Serotonin 5-HT2 Receptor Antagonists - pharmacology

Rats, Sprague-Dawley

Receptors, Serotonin - metabolism

Serotonergic Neurons - metabolism

Serotonin Receptor Agonists - pharmacology

Animals

Respiratory Mechanics - physiology

Hypercapnia - metabolism

Details

Title: Subtitle: Serotonergic mechanisms are necessary for central respiratory chemoresponsiveness in situ
Creators: Andrea E Corcoran - Institute of Arctic Biology and Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK 99775, USA. andrea.e.corcoran@dartmouth.edu
George B Richerson
Michael B Harris
Resource Type: Journal article
Publication Details: Respiratory physiology & neurobiology, Vol.186(2), pp.214-220
DOI: 10.1016/j.resp.2013.02.015
PMID: 23454177
PMCID: PMC3645885
NLM abbreviation: Respir Physiol Neurobiol
ISSN: 1569-9048
eISSN: 1878-1519
Publisher: Netherlands
Grant note: U54NS041069-06A1 / NINDS NIH HHS U54 NS041069 / NINDS NIH HHS R01 HD052772 / NICHD NIH HHS P01 HD036379 / NICHD NIH HHS P01HD36379 / NICHD NIH HHS R01HD052772 / NICHD NIH HHS
Language: English
Date published: 04/01/2013
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Neurosurgery
Record Identifier: 9984020753702771

Metrics

10 Record Views

30 Times Cited - Web of Science