Journal article
Serum HSP27 is associated with medullary perfusion in kidney allografts
Journal of nephrology, Vol.25(6), pp.1075-1080
11/01/2012
DOI: 10.5301/jn.5000099
PMCID: PMC4609193
PMID: 22383348
Abstract
Background: Heat shock protein 27 (HSP27) is a small HSP up-regulated in response to stress in the kidney. The relationship between HSP27 and intrarenal oxygenation in patients with native and transplant kidney disease is unknown.
Methods: We compared HSP27 levels, intrarenal oxygenation measured by blood oxygen-level dependent (BOLD) imaging using R2* values, and perfusion determined by arterial spin labeling (ASL) magnetic resonance imaging (MRI), between patients with native and transplant kidney disease (n=28).
Results: There were no statistical differences in mean age (53.9 vs. 47.1 years), kidney function (63.6 vs. 50.7 ml/min per 1.73 m(2)), mean arterial blood pressure (91.6 vs. 91.1 mm Hg), hematocrit (40.6% vs. 39.3%), diuretic or angiotensin-converting enzyme inhibitor use, serum or urine levels of hydrogen peroxide, nitric oxide, F-2 isoprostanes and HSP27 between native and transplant kidneys. BOLD-MRI studies demonstrated comparable patterns in intrarenal oxygen bioavailability (medullary R-2* 18.1 vs. 18.3/s and cortical R-2* 12 vs. 11.7/s, respectively). However, medullary perfusion was significantly lower in transplant kidneys (36.4 vs. 78.7 m1/100 g per minute, p=0.0002). There was a linear relationship between serum HSP27 concentrations and medullary perfusion in kidney allografts (HSP27 concentration [ng/mL] = 0.78 + 0.09 medullary perfusion, R-2=0.43, p=0.01).
Conclusions: Our study demonstrates that medullary perfusion is significantly lower in kidney allografts compared with native kidneys with comparable renal function. We further noted a direct association between serum HSP27 levels and medullary perfusion after transplantation. Additional studies are needed to examine the role of HSP27 as a biomarker of kidney disease progression.
Details
- Title: Subtitle
- Serum HSP27 is associated with medullary perfusion in kidney allografts
- Creators
- Eva Marquez - Hospital Del MarElizabeth Sadowski - University of Wisconsin–MadisonShannon Reese - University of Wisconsin–MadisonAparna Vidyasagar - University of Wisconsin–MadisonNathan Artz - University of Wisconsin–MadisonSean Fain - University of Wisconsin–MadisonLynn Jacobson - University of Wisconsin–MadisonWilliam Swain - University of Wisconsin–MadisonArjang Djamali - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- Journal of nephrology, Vol.25(6), pp.1075-1080
- DOI
- 10.5301/jn.5000099
- PMID
- 22383348
- PMCID
- PMC4609193
- NLM abbreviation
- J Nephrol
- ISSN
- 1121-8428
- eISSN
- 1724-6059
- Publisher
- Springer Nature
- Number of pages
- 6
- Grant note
- R01 DK 073680; R21 DK070243 / National Institutes of Health, NIDDK grants R21DK070243 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) American Society of Nephrology-American Society of Transplantation John Merrill Award
- Language
- English
- Date published
- 11/01/2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Health, Sport, and Human Physiology
- Record Identifier
- 9984275054802771
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