Severe acute respiratory syndrome coronavirus protein 6 is required for optimal replication

Jincun Zhao; Ana Falcón; Haixia Zhou; Jason Netland; Luis Enjuanes; Pilar Pérez Breña; Stanley Perlman

doi:10.1128/JVI.02371-08

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Severe acute respiratory syndrome coronavirus protein 6 is required for optimal replication

Journal article

Open access

Peer reviewed

Severe acute respiratory syndrome coronavirus protein 6 is required for optimal replication

Jincun Zhao, Ana Falcón, Haixia Zhou, Jason Netland, Luis Enjuanes, Pilar Pérez Breña and Stanley Perlman

Journal of virology, Vol.83(5), pp.2368-2373

03/2009

DOI: 10.1128/JVI.02371-08

PMCID: PMC2643704

PMID: 19091867

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https://europepmc.org/articles/pmc2643704View

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Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) encodes several accessory proteins of unknown function. One of these proteins, protein 6 (p6), which is encoded by ORF6, enhances virus replication when introduced into a heterologous murine coronavirus (mouse hepatitis virus [MHV]) but is not essential for optimal SARS-CoV replication after infection at a relatively high multiplicity of infection (MOI). Here, we reconcile these apparently conflicting results by showing that p6 enhances SARS-CoV replication to nearly the same extent as when expressed in the context of MHV if cells are infected at a low MOI and accelerates disease in mice transgenic for the human SARS-CoV receptor.

Cell Line

Severe Acute Respiratory Syndrome - virology

Receptors, Virus - metabolism

Open Reading Frames

Mice, Transgenic

SARS Virus - metabolism

Viral Proteins - metabolism

SARS Virus - pathogenicity

STAT1 Transcription Factor - metabolism

Animals

Virus Replication

Mice

SARS Virus - physiology

Interferon-gamma - pharmacology

Details

Title: Subtitle: Severe acute respiratory syndrome coronavirus protein 6 is required for optimal replication
Creators: Jincun Zhao - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
Ana Falcón
Haixia Zhou
Jason Netland
Luis Enjuanes
Pilar Pérez Breña
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.83(5), pp.2368-2373
DOI: 10.1128/JVI.02371-08
PMID: 19091867
PMCID: PMC2643704
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: P01 AI060699 / NIAID NIH HHS
Language: English
Date published: 03/2009
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777346602771

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