Journal article
Sevoflurane-induced ionic current in acutely dissociated CA1 pyramidal neurons of the rat hippocampus
Brain research, Vol.645(1), pp.303-308
1994
DOI: 10.1016/0006-8993(94)91664-0
PMID: 8062091
Abstract
The electrophysiological properties of sevoflurane (Sev)-induced current (
I
Sev) were investigated in CA1 pyramidal neurons freshly dissociated from the rat hippocampus by using the nystatin perforated patch recording configuration under voltag-clamp condition. Within the range of Sev concentrations from 3 · 10
−4 to 2 · 10
−3 M,
I
sev was an inward current which consisted of an initial transient peak component and a successive steady-state plateau component. The peak current component increased in a concentration-dependent manner with a conductance increase. The application of Sev over 2 · 10
−3 M, however, suppressed the peak and steady-state current components with a concomitant decrease in conductance and elicited a transient inward current (‘hump’ current) immediately after wash out. The current-voltage relationship for
I
Sev showed some outward rectification suggesting a slight voltage-dependency of the
I
sev. The reversal potential of
I
Sev (
E
Sev) was close to the
E
Cl and shifted by 52 mV for a 10-fold change in extracellular Cl
− concentrations, indicating that
I
Sev is passing through Cl
− channels. The single channel conductance obtained from the analysis of the variance of
I
Sev fluctuations was 15.3 ± 1.3pS.
Details
- Title: Subtitle
- Sevoflurane-induced ionic current in acutely dissociated CA1 pyramidal neurons of the rat hippocampus
- Creators
- Jie WuNobutoshi HarataNorio Akaike
- Resource Type
- Journal article
- Publication Details
- Brain research, Vol.645(1), pp.303-308
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0006-8993(94)91664-0
- PMID
- 8062091
- ISSN
- 0006-8993
- eISSN
- 1872-6240
- Grant note
- DOI: 10.13039/501100001700, name: Ministry of Education, Culture, Sports, Science and Technology
- Language
- English
- Date published
- 1994
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984025577902771
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