Journal article
Sex Differences in Oxidative Stress–Mediated Reductions in Microvascular Endothelial Function in Young Adult e-Cigarette Users
Hypertension (Dallas, Tex. 1979), Vol.80(12), pp.2641-2649
12/2023
DOI: 10.1161/HYPERTENSIONAHA.123.21684
PMCID: PMC10848654
PMID: 37800370
Abstract
Background: Chronic e-cigarette (EC) use is reported to decrease vascular endothelial function. However, the mechanism(s) mediating this reduction remain unclear. In this study, we examined endothelium- and NO-dependent dilation, and the role of oxidative stress in attenuating these responses, in healthy young EC users (n=20, 10 males/10 females) compared with healthy controls (n=20, 10 males/10 females). We hypothesized that EC would have reduced endothelium- and NO-dependent dilation and administration of the superoxide scavenger tempol would increase these responses in EC. We further hypothesized that female EC would have the greatest reductions in endothelium- and NO-dependent dilation. Methods: We assessed microvascular endothelium-dependent vasodilator function in vivo by measurement of cutaneous vascular conductance (%CVCmax) responses to a standardized local heating protocol in control and 10 μM tempol-treated sites. After full expression of the local heating response, 15 mM N G -nitro-L-arginine methyl ester (NO synthase inhibition) was perfused. Results: EC had significantly reduced endothelium- (73±15 versus 87±9%CVCmax; P <0.001) and NO-dependent (48±17% versus 62±15%; P =0.011) dilation. Tempol perfusion increased endothelium-dependent (84±12%CVCmax P =0.01) and NO-dependent (63±14% P =0.005) dilation in EC but had no effect in healthy control. Within female sex, EC had lower endothelium-dependent (71±13 versus 89±7%CVCmax; P =0.002) and NO-dependent (50±6 versus 69±11%; P =0.005) dilation compared with healthy control, and tempol augmented endothelium-dependent (83±13%CVCmax; P =0.002) and NO-dependent (62±13%; P =0.015) dilation. There were no group or treatment differences within male sex. Conclusion: Healthy young adult EC users have reduced microvascular endothelium-dependent and NO-dependent dilation, driven by greater reductions in female EC users, and mediated in part by superoxide.
Details
- Title: Subtitle
- Sex Differences in Oxidative Stress–Mediated Reductions in Microvascular Endothelial Function in Young Adult e-Cigarette Users
- Creators
- Kristen M. Halstead - University of IowaElizabeth M. Wetzel - University of IowaJosalyn L. Cho - Department of Internal Medicine, Carver College of Medicine, Iowa City, Iowa (J.L.C.)Anna E. Stanhewicz - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Hypertension (Dallas, Tex. 1979), Vol.80(12), pp.2641-2649
- DOI
- 10.1161/HYPERTENSIONAHA.123.21684
- PMID
- 37800370
- PMCID
- PMC10848654
- NLM abbreviation
- Hypertension
- ISSN
- 0194-911X
- eISSN
- 1524-4563
- Language
- English
- Electronic publication date
- 10/06/2023
- Date published
- 12/2023
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Fraternal Order of Eagles Diabetes Research Center; Health, Sport, and Human Physiology ; Internal Medicine
- Record Identifier
- 9984475075802771
Metrics
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