Journal article
Signal Sequences Initiate the Pathway of Maturation in the Endoplasmic Reticulum Lumen
The Journal of biological chemistry, Vol.278(32), pp.30365-30372
08/08/2003
DOI: 10.1074/jbc.M302117200
PMID: 12771148
Abstract
An interaction between an N-terminal signal sequence and the translocon leads to the initiation of protein translocation into the endoplasmic reticulum lumen. Subsequently, folding and modification of the substrate rapidly ensue. The close temporal coordination of these processes suggests that they may be structurally and functionally coordinated as well. Here we show that information encoded in the hydrophobic domain of a signal sequence influences the timing and efficiency of at least two steps in maturation, namely N-linked glycosylation and signal sequence cleavage. We demonstrate that these consequences correlate with and likely stem from the nature of the initial association made between the signal sequence and the translocon during the initiation of translocation. We propose a model by which these maturational events are controlled by the signal sequence-translocon interaction. Our work demonstrates that the pathway taken by a nascent chain through post-translational maturation depends on information encoded in its signal sequence.
Details
- Title: Subtitle
- Signal Sequences Initiate the Pathway of Maturation in the Endoplasmic Reticulum Lumen
- Creators
- D.Thomas Rutkowski - Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94143Carolyn M Ott - Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94143Jon R Polansky - Ophthalmology, University of California San Francisco, San Francisco, California 94143Vishwanath R Lingappa - Department of Physiology and Medicine, University of California San Francisco, San Francisco, California 94143
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.278(32), pp.30365-30372
- DOI
- 10.1074/jbc.M302117200
- PMID
- 12771148
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 08/08/2003
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984094320602771
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