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Signal transduction pathways in constriction of the basilar artery in vivo
Journal article   Open access   Peer reviewed

Signal transduction pathways in constriction of the basilar artery in vivo

M A Murray, F M Faraci and D D Heistad
Hypertension (Dallas, Tex. 1979), Vol.19(6 Pt 2), pp.739-742
06/1992
DOI: 10.1161/01.HYP.19.6.739
PMID: 1592475
url
https://doi.org/10.1161/01.HYP.19.6.739View
Published (Version of record) Open Access

Abstract

We examined effects of a putative myosin light chain kinase inhibitor in the cerebral circulation in vivo. In anesthetized rats, diameter of basilar arteries was measured through a cranial window (control, 232 +/- 10 microns, mean +/- SEM). Vessel diameter was measured during topical application of agonists and antagonists. ML-7, which has been reported to compete with adenosine triphosphate for binding to the catalytic site on myosin light chain kinase, attenuated vasoconstriction in response to prostaglandin F2 alpha (10(-6) M; -22 +/- 1% before versus -14 +/- 1% and -3 +/- 2% during ML-7, 10(-7) and 10(-6) M, respectively; p less than 0.05). ML-7 (10(-6) M) did not affect baseline diameter. Responses to serotonin (10(-8) M) and phorbol 12,13-dibutyrate (10(-8) M) were not attenuated by ML-7. Thus, constriction of the basilar artery induced by prostaglandin F2 alpha in vivo is attenuated by an inhibitor of myosin light chain kinase.
Basilar Artery - drug effects Rats Dimethyl Sulfoxide - pharmacology Male Myosin-Light-Chain Kinase - antagonists & inhibitors Rats, Inbred Strains Vasoconstriction - drug effects Naphthalenes - pharmacology Azepines - pharmacology Vasoconstriction - physiology Animals Signal Transduction - physiology Basilar Artery - physiology Dinoprost - pharmacology Pharmaceutical Vehicles

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