Logo image
Back
Signaling to a CD5+ B-cell clone through surface Ig and MHC class II molecules
Journal article   Peer reviewed

Signaling to a CD5+ B-cell clone through surface Ig and MHC class II molecules

G A Bishop
Annals of the New York Academy of Sciences, Vol.651(1), pp.228-240
05/04/1992
DOI: 10.1111/j.1749-6632.1992.tb24618.x
PMID: 1376042

View Online

Abstract

Cells of the CD5+ mouse B-cell clone CH12.LX are induced to become antibody-secreting cells by costimulatory signals delivered by binding of their surface Ig and MHC class II molecules. Class II-mediated signals can be delivered by the binding of either T cells or class II-specific monoclonal antibodies. Divalent, but not monovalent antigen-binding fragments of mAbs are effective in signalling, and cross-linking intact anti-class II mAbs with isotype-specific Ab enhance class II-mediated signaling. Class II-mediated signaling is accompanied by a rise in cAMP and is blocked by an adenyl cyclase inhibitor. The cAMP analogue dibutyryl cAMP, can partially but not completely substitute for the class II-mediated signal. The costimulatory Ig-mediated signal can be delivered by binding of either antigen or antiidiotype Ab, but anti-IgM Abs are much less effective. Antibodies specific for Ig constant regions are much more effective, however, if they engage both the mIgM and mIgD molecules; anti-kappa Abs or a combination of anti-IgM and anti-IgD Abs were more effective in signaling.
 Dideoxyadenosine - pharmacology Antigens, CD - immunology Signal Transduction Adenylyl Cyclases - metabolism Antibodies, Monoclonal Animals CD5 Antigens Cell Differentiation - drug effects Lymphoma, B-Cell - immunology B-Lymphocyte Subsets - immunology Receptors, Antigen, B-Cell - physiology Clone Cells Mice Histocompatibility Antigens Class II - physiology Tumor Cells, Cultured Cyclic AMP - metabolism

Details

Metrics

20 Record Views
Logo image