Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina

Natalie R Leach; Richard J Roller

doi:10.1016/j.virol.2010.07.002

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Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina

Journal article

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Peer reviewed

Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina

Natalie R Leach and Richard J Roller

Virology (New York, N.Y.), Vol.406(1), pp.127-137

10/10/2010

DOI: 10.1016/j.virol.2010.07.002

PMCID: PMC2948959

PMID: 20674954

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Abstract

The nuclear lamina is thought to be a steric barrier to the herpesvirus capsid. Disruption of the lamina accompanied by phosphorylation of lamina proteins is a conserved feature of herpesvirus infection. In HSV-1-infected cells, protein kinase C (PKC) alpha and delta isoforms are recruited to the nuclear membrane and PKC delta has been implicated in phosphorylation of emerin and lamin B. We tested two critical hypotheses about the mechanism and significance of lamina disruption. First, we show that chemical inhibition of all PKC isoforms reduced viral growth five-fold and inhibited capsid egress from the nucleus. However, specific inhibition of either conventional PKCs or PKC delta does not inhibit viral growth. Second, we show hyperphosphorylation of emerin by viral and cellular kinases is required for its disassociation from the lamina. These data support hypothesis that phosphorylation of lamina components mediates lamina disruption during HSV nuclear egress.

nuclear lamina

HSV-1

nuclear egress

emerin

protein kinase C

Details

Title: Subtitle: Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina
Creators: Natalie R Leach - Department of Microbiology, University of Iowa, Iowa City, IA 52242
Richard J Roller - Department of Microbiology, University of Iowa, Iowa City, IA 52242
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.406(1), pp.127-137
DOI: 10.1016/j.virol.2010.07.002
PMID: 20674954
PMCID: PMC2948959
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Grant note: name: PHS, award: AI 41478; name: PHS, award: T32 AI 007533
Language: English
Date published: 10/10/2010
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001203002771

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