Journal article
Significance of the centrally expressed TRP channel painless in Drosophila courtship memory
Learning & memory (Cold Spring Harbor, N.Y.), Vol.20(1), pp.34-40
01/2013
DOI: 10.1101/lm.029041.112
PMCID: PMC3533128
PMID: 23247253
Abstract
Considerable evidence has demonstrated that transient receptor potential (TRP) channels play vital roles in sensory neurons, mediating responses to various environmental stimuli. In contrast, relatively little is known about how TRP channels exert their effects in the central nervous system to control complex behaviors. This is also true for the
Drosophila
TRP channel encoded by
painless
(
pain
). The Pain TRP channel is expressed in a subset of sensory neurons and involved in behavioral responses to thermal, chemical, and mechanical stimuli. Its physiological roles in brain neurons, however, remain largely elusive. Using multiple mutant alleles and tranformants for
pain
, here we demonstrate that the brain-expressed Pain TRP channel is required for long-term memory (LTM), but not for short-lasting memory, induced by courtship conditioning in adult males. The courtship LTM phenotype in
pain
mutants was rescued by expressing wild-type
pain
temporarily, prior to conditioning, in adult flies. In addition, targeted expression of
pain
RNAi in either the mushroom bodies (MBs) or insulin-producing cells (IPCs) resulted in defective courtship LTM. These results indicate that the Pain TRP channels in the MBs and IPCs control neuronal plasticity that is required for the formation of a certain type of long-lasting associative memory in
Drosophila
.
Details
- Title: Subtitle
- Significance of the centrally expressed TRP channel painless in Drosophila courtship memory
- Creators
- Takaomi Sakai - Department of Biological Sciences, Tokyo Metropolitan University, Hachi-oji 192-0397, Tokyo, JapanShoma Sato - Department of Biological Sciences, Tokyo Metropolitan University, Hachi-oji 192-0397, Tokyo, JapanHiroshi Ishimoto - Department of Anesthesia, University of Iowa, Iowa City, Iowa 52242, USAToshihiro Kitamoto - Department of Anesthesia, University of Iowa, Iowa City, Iowa 52242, USA
- Resource Type
- Journal article
- Publication Details
- Learning & memory (Cold Spring Harbor, N.Y.), Vol.20(1), pp.34-40
- Publisher
- Cold Spring Harbor Laboratory Press
- DOI
- 10.1101/lm.029041.112
- PMID
- 23247253
- PMCID
- PMC3533128
- ISSN
- 1072-0502
- eISSN
- 1549-5485
- Alternative title
- Drosophila painless in courtship memory
- Language
- English
- Date published
- 01/2013
- Academic Unit
- Iowa Neuroscience Institute; Anesthesia; Neuroscience and Pharmacology
- Record Identifier
- 9984006464002771
Metrics
22 Record Views