Journal article
Silica Increases Tumor Necrosis Factor (TNF) Production, in Part, by Upregulating the TNF Promoter
Experimental lung research, Vol.20(6), pp.613-625
1994
DOI: 10.3109/01902149409031740
PMID: 7882909
Abstract
Silica causes release of tumor necrosis factor (TNF) from mononuclear phagocytes. One hypothesis is that silica increases TNF production, in part, by upregulating the TNF gene. To evaluate this hypothesis, THP-1 cells (a myelomonocytic cell line) were exposed to various amounts of silica and then the TNF gene transcription was evaluated. In this study silica caused a dose-dependent increase in TNF mRNA and the peak response occurred at 3 h following stimulation. A transient transfection assay also showed that silica upregulated expression of a TNF CAT construct in THP-1 cells. Furthermore, a nuclear run-on assay demonstrated that silica particles induce increased TNF gene transcription in exposed cells. THP-1 cells cultured for various periods of time in the presence of silica released TNF into the cell supernatants. These studies show that silica can up-regulate the TNF gene, which results in the release of TNF protein from the cells.
Details
- Title: Subtitle
- Silica Increases Tumor Necrosis Factor (TNF) Production, in Part, by Upregulating the TNF Promoter
- Creators
- Dana Savici - 1Pulmonary Division, Department of Medicine, Veterans Affairs Administration Medical Center, and University of Iowa College of Medicine, Iowa City, Iowa, USABei He - 1Pulmonary Division, Department of Medicine, Veterans Affairs Administration Medical Center, and University of Iowa College of Medicine, Iowa City, Iowa, USALois J Geist - 1Pulmonary Division, Department of Medicine, Veterans Affairs Administration Medical Center, and University of Iowa College of Medicine, Iowa City, Iowa, USAMartha M Monick - 1Pulmonary Division, Department of Medicine, Veterans Affairs Administration Medical Center, and University of Iowa College of Medicine, Iowa City, Iowa, USAGary W Hunninghake - 1Pulmonary Division, Department of Medicine, Veterans Affairs Administration Medical Center, and University of Iowa College of Medicine, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Experimental lung research, Vol.20(6), pp.613-625
- Publisher
- Informa UK Ltd
- DOI
- 10.3109/01902149409031740
- PMID
- 7882909
- ISSN
- 0190-2148
- eISSN
- 1521-0499
- Language
- English
- Date published
- 1994
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Provost Office Administration; Internal Medicine
- Record Identifier
- 9984094648402771
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