Journal article
Silicalite nanoparticles that promote transgene expression
Nanotechnology, Vol.19(17), pp.175103-175103 (7)
04/30/2008
DOI: 10.1088/0957-4484/19/17/175103
PMID: 21825661
Abstract
Here, we report on a new zeolite-based silicalite nanoparticle that can enhance the transfection efficiencies generated by poly ethylene imine-plasmid DNA (PEI-pDNA) complexes via a sedimentation mechanism and can enhance the transfection efficiencies of pDNA alone when surface functionalized with amine groups. The silicalite nanoparticles have a mean size of 55 nm. Functionalizing the silicalite nanoparticles with amine groups results in a clear transition in zeta potential from -25.9 ± 2.3 mV (pH 7.4) for unfunctionalized silicalite nanoparticles to 4.9 ± 0.7 mV (pH 7.4) for amine functionalized silicalite nanoparticles. We identify that silicalite nanoparticles used to promote non-viral vector acceleration to the cell surface are found in acidic vesicles or the cytoplasm but not the nucleus. An MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay showed that the silicalite nanoparticles were non-toxic at the concentrations tested for transfection. We show that surface functionalization of silicalite nanoparticles with amine groups results in a significant (230%) increase in transfection efficiency of pDNA when compared to unfunctionalized silicalite nanoparticles. Silicalite nanoparticles enhanced pDNA-PEI induced transfection of human embryonic kidney (HEK-293) cells by over 150%.
Details
- Title: Subtitle
- Silicalite nanoparticles that promote transgene expression
- Creators
- Megan E Pearce - Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USAHoang Q MaiNamhoon LeeSarah C LarsenAliasger K Salem
- Resource Type
- Journal article
- Publication Details
- Nanotechnology, Vol.19(17), pp.175103-175103 (7)
- DOI
- 10.1088/0957-4484/19/17/175103
- PMID
- 21825661
- NLM abbreviation
- Nanotechnology
- ISSN
- 0957-4484
- eISSN
- 1361-6528
- Publisher
- England
- Language
- English
- Date published
- 04/30/2008
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Chemistry; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983986693702771
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