Journal article
Simian TT virus (s-TTV) infection in patients with liver diseases
Hepatology research, Vol.25(2), pp.135-142
2003
DOI: 10.1016/S1386-6346(02)00250-4
PMID: 12644049
Abstract
Recently, we identified TTV isolates from nonhuman primates and named them simian TTV (s-TTV). To investigate the prevalence of s-TTV in humans, we examined sera from healthy individuals and patients with liver diseases in Japan for the presence of s-TTV DNA by PCR assay. s-TTV DNA was determined by nested PCR using s-TTV-specific primers designed from untranslated region of s-TTV genome. s-TTV DNA sequence was detected in three of 200 (1.5%) healthy adults but none of 48 infants without liver disease. On the other hand, s-TTV DNA was detected in 30 of 287 (10.5%) Japanese patients with liver disease. s-TTV coinfection with hepatitis B virus and hepatitis C virus were present in 16.7 and 30% of these patients, respectively, while 53.3% of patients were positive for s-TTV alone. Nucleotide sequence analyses in 20 patients confirmed that these PCR products were derived from s-TTV genome sequences and nearly 85% identical to those of s-TTV prototypes from chimpanzees. Phylogenetic analysis demonstrated that all s-TTV isolates from humans were distinguished clearly from the human TTV isolates. Furthermore, s-TTV in humans was classified into two different genotypes as well as simians. Our results indicate that generally 10.5% of Japanese patients with liver diseases were infected with s-TTV. The routes of s-TTV transmission from animal to human require clarification.
Details
- Title: Subtitle
- Simian TT virus (s-TTV) infection in patients with liver diseases
- Creators
- Yoko Iwaki - Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, JapanNaoto Aiba - Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, JapanHuy Thien Tuan Tran - Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, JapanXin Ding - Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, JapanShigeki Hayashi - Division of Gastroenterology, International Medical Center of Japan, Tokyo, JapanYasuyuki Arakawa - 3rd Department of Internal Medicine, Nihon University School of Medicine, Tokyo, JapanTetsutaro Sata - Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, JapanKenji Abe - Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan
- Resource Type
- Journal article
- Publication Details
- Hepatology research, Vol.25(2), pp.135-142
- Publisher
- Elsevier Ireland Ltd
- DOI
- 10.1016/S1386-6346(02)00250-4
- PMID
- 12644049
- ISSN
- 1386-6346
- eISSN
- 1872-034X
- Language
- English
- Date published
- 2003
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094628102771
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