Journal article
Simple and reproducible approaches for the collection of select porcine ganglia
Journal of neuroscience methods, Vol.289, pp.93-98
09/01/2017
DOI: 10.1016/j.jneumeth.2017.06.005
PMCID: PMC5582004
PMID: 28602889
Abstract
•Reproducible and rapid techniques for isolating three common porcine ganglia.•Image-guided methods to accommodate novice to experienced audiences.•Methods emphasize preservation of ganglia structure for morphometric examination.•Dual approaches for DRG collection depending on anticipated downstream uses.
The anatomy and physiology of the pig nervous system is more similar to humans compared to traditional rodent models. This makes the pig an attractive model to answer questions relating to human health and disease. Yet the technical and molecular tools available to pig researchers are limited compared to rodent researchers.
We developed simple and rapid methods to isolate the trigeminal, nodose (distal vagal), and dorsal root ganglia from neonatal pigs. We selected these ganglia due to their broad applicability to basic science researchers and clinicians.
Use of these methods resulted in reproducible isolation of all three types of ganglia as validated by histological examination.
There are currently no methods that describe a step-by-step protocol to isolate these porcine ganglia.
In conclusion, these methods for ganglia collection will facilitate and accelerate future neuroscience investigations in pig models of human disease.
Details
- Title: Subtitle
- Simple and reproducible approaches for the collection of select porcine ganglia
- Creators
- David K Meyerholz - Department of Pathology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USALeah R Reznikov - Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA
- Resource Type
- Journal article
- Publication Details
- Journal of neuroscience methods, Vol.289, pp.93-98
- DOI
- 10.1016/j.jneumeth.2017.06.005
- PMID
- 28602889
- PMCID
- PMC5582004
- NLM abbreviation
- J Neurosci Methods
- ISSN
- 0165-0270
- eISSN
- 1872-678X
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: P01 HL091842, P30 DK054759, R00 HL119560-03, P01 HL051670; DOI: 10.13039/100000897, name: Cystic Fibrosis Foundation, award: P01 HL091842, P30 DK054759, R00 HL119560-03, P01 HL051670
- Language
- English
- Date published
- 09/01/2017
- Academic Unit
- Pathology
- Record Identifier
- 9984083872702771
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