Simplified and versatile method for isolation of high-quality RNA from pancreas

Michelle GRIFFIN; Maisam ABU-EL-HAIJA; Marwa ABU-EL-HAIJA; Tatiana ROKHLINA; Aliye UC

doi:10.2144/0000113862

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Simplified and versatile method for isolation of high-quality RNA from pancreas

Journal article

Open access

Peer reviewed

Simplified and versatile method for isolation of high-quality RNA from pancreas

Michelle GRIFFIN, Maisam ABU-EL-HAIJA, Marwa ABU-EL-HAIJA, Tatiana ROKHLINA and Aliye UC

BioTechniques, Vol.52(5), pp.332-334

2012

DOI: 10.2144/0000113862

PMID: 22578126

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Abstract

Isolation of high-quality RNA from pancreas is challenging because the organ contains large quantities of RNases and undergoes autolysis upon harvest. Here we present a simplified perfusion method of the pancreas using an RNase inhibitor. The technique consistently yields high-quality RNA from stored pancreas samples suitable for molecular biology applications, including quantitative RT-PCR. Yields are comparable to RNA isolated from pancreas immediately, but superior to RNA isolated from stored samples that were snap-frozen or immersed in an RNase inhibitor solution. In addition, when compared to the previously reported in situ ductal perfusion technique, our method does not cause histological artifacts.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Diverse techniques

Molecular and cellular biology

Details

Title: Subtitle: Simplified and versatile method for isolation of high-quality RNA from pancreas
Creators: Michelle GRIFFIN - Departments of Pediatrics and, University of Iowa Carver College of Medicine, Iowa City, IA, United States
Maisam ABU-EL-HAIJA - Departments of Pediatrics and, University of Iowa Carver College of Medicine, Iowa City, IA, United States
Marwa ABU-EL-HAIJA - Departments of Pediatrics and, University of Iowa Carver College of Medicine, Iowa City, IA, United States
Tatiana ROKHLINA - Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA, United States
Aliye UC - Departments of Pediatrics and, University of Iowa Carver College of Medicine, Iowa City, IA, United States
Resource Type: Journal article
Publication Details: BioTechniques, Vol.52(5), pp.332-334
DOI: 10.2144/0000113862
PMID: 22578126
NLM abbreviation: Biotechniques
ISSN: 0736-6205
eISSN: 1940-9818
Publisher: Eaton; Natick, MA
Language: English
Date published: 2012
Academic Unit: Stead Family Department of Pediatrics; Radiation Oncology; Gastroenterology, Hepatology, Pancreatology, and Nutrition
Record Identifier: 9984047678702771

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