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Simultaneous Intranasal/Intravascular Antibody Labeling of CD4+ T Cells in Mouse Lungs
Journal article   Open access   Peer reviewed

Simultaneous Intranasal/Intravascular Antibody Labeling of CD4+ T Cells in Mouse Lungs

Yanqun Wang, Jing Sun, Rudragouda Channappanavar, Jingxian Zhao, Stanley Perlman and Jincun Zhao
Bio-protocol, Vol.7(1), e2099
01/05/2017
DOI: 10.21769/BioProtoc.2099
PMCID: PMC5431592
PMID: 28516121
url
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431592View
Published (Version of record) Open Access

Abstract

CD4 + T cell responses have been shown to be protective in many respiratory virus infections. In the respiratory tract, CD4 + T cells include cells in the airway and parenchyma and cells adhering to the pulmonary vasculature. Here we discuss in detail the methods that are useful for characterizing CD4 + T cells in different anatomic locations in mouse lungs.
Flow Cytometry intravascular antibody labeling Bronchoalveolar lavage fluid Intranasal Lung Memory CD4+ T cell

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