Journal article
Simultaneous Measurements of Glucose, Glutamine, Ammonia, Lactate, and Glutamate in Aqueous Solutions by Near-Infrared Spectroscopy
Applied spectroscopy, Vol.50(2), pp.270-276
02/1996
DOI: 10.1366/0003702963906447
Abstract
Calibration models are generated and evaluated for the measurement of five different components in synthetic mixtures prepared in aqueous solutions. Mixtures of glucose, glutamine, ammonia, lactate, and glutamate were prepared to simulate concentration levels expected during routine bioreactor fermentation processes. Near-IR spectra were collected from these solutions over the spectral range from 5000 to 4000 cm−1. This spectral information was used to build individual multivariate calibration models for each analyte. Models were constructed on the basis of partial least-squares regression of raw and Fourier filtered absorbance spectra. Each analyte could be detected selectively with mean percent errors of prediction ranging from 4 to 8%.
Details
- Title: Subtitle
- Simultaneous Measurements of Glucose, Glutamine, Ammonia, Lactate, and Glutamate in Aqueous Solutions by Near-Infrared Spectroscopy
- Creators
- Hoeil Chung - Department of Chemistry, (H.C., M.A.A.) and Department of Chemical and Biochemical Engineering (M.R., D.W.M.), University of Iowa, Iowa City, Iowa 52242Mark A Arnold - Department of Chemistry, (H.C., M.A.A.) and Department of Chemical and Biochemical Engineering (M.R., D.W.M.), University of Iowa, Iowa City, Iowa 52242Martin Rhiel - Department of Chemistry, (H.C., M.A.A.) and Department of Chemical and Biochemical Engineering (M.R., D.W.M.), University of Iowa, Iowa City, Iowa 52242David W Murhammer - Department of Chemistry, (H.C., M.A.A.) and Department of Chemical and Biochemical Engineering (M.R., D.W.M.), University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Applied spectroscopy, Vol.50(2), pp.270-276
- Publisher
- SAGE Publications; London, England
- DOI
- 10.1366/0003702963906447
- ISSN
- 0003-7028
- eISSN
- 1943-3530
- Language
- English
- Date published
- 02/1996
- Academic Unit
- Chemical and Biochemical Engineering; Chemistry; Center for Biocatalysis and Bioprocessing
- Record Identifier
- 9984003415902771
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