Journal article
Simultaneous assessment of antigen-stimulated cytokine production and memory subset composition of memory CD8 T cells
Journal of immunological methods, Vol.313(1), pp.161-168
2006
DOI: 10.1016/j.jim.2006.04.005
PMID: 16762359
Abstract
The functional identification of antigen-specific CD8 T cell populations is critical to understanding host responses to infection by intracellular pathogens. Furthermore, assessing the properties of protective memory CD8 T cell populations generated by immunization is necessary in the rational design of vaccines. Recently, a classification scheme was proposed in which memory CD8 T cells were divided into one of two distinct subsets, based on CD62L expression, that have different functional properties and protective capacities.
Intracellular cytokine staining functionally identifies antigen-specific CD8 T cell populations after short in vitro stimulation with cognate peptide. This short stimulation, however, results in the cleavage of CD62L from the cell surface of antigen-specific CD8 T cells and precludes distinguishing CD62L
hi- and CD62L
lo-expressing memory cell subsets within this population. Here, we describe a method of preserving CD62L expression by the antigen-specific CD8 T cell population during coculture with antigen. This methodology allows for the identification and functional assessment of antigen-specific memory CD8 T cell populations, while simultaneously characterizing the memory subset composition of that population. Using this method, we directly identify differences in IL-2 production capacity by CD62L
hi- and CD62L
lo-expressing antigen-specific memory CD8 T cell populations.
Details
- Title: Subtitle
- Simultaneous assessment of antigen-stimulated cytokine production and memory subset composition of memory CD8 T cells
- Creators
- Ali Jabbari - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA, USAJohn T Harty - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Journal of immunological methods, Vol.313(1), pp.161-168
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jim.2006.04.005
- PMID
- 16762359
- ISSN
- 0022-1759
- eISSN
- 1872-7905
- Language
- English
- Date published
- 2006
- Academic Unit
- Dermatology; Pathology
- Record Identifier
- 9984025344102771
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