Simultaneous determination of lamotrigine, zonisamide, and carbamazepine in human plasma by high-performance liquid chromatography

Elizabeth Greiner-Sosanko; Darla R Lower; Mohamed A Virji; Matthew D Krasowski

doi:10.1002/bmc.753

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Simultaneous determination of lamotrigine, zonisamide, and carbamazepine in human plasma by high-performance liquid chromatography

Journal article

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Simultaneous determination of lamotrigine, zonisamide, and carbamazepine in human plasma by high-performance liquid chromatography

Elizabeth Greiner-Sosanko, Darla R Lower, Mohamed A Virji and Matthew D Krasowski

Biomedical chromatography, Vol.21(3), pp.225-228

03/2007

DOI: 10.1002/bmc.753

PMCID: PMC2231333

PMID: 17230449

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https://www.ncbi.nlm.nih.gov/pmc/articles/2231333View

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Abstract

A high-performance liquid chromatography assay with ultraviolet detection was developed for the simultaneous determination of the anti-epileptic drugs lamotrigine, carbamazepine and zonisamide in human plasma and serum. Lamotrigine, carbamazepine, zonisamide and the internal standard chloramphenicol were extracted from serum or plasma using liquid-liquid extraction under alkaline conditions into an organic solvent. The method was linear in the range 1-30 microg/mL for lamotrigine, 2-20 microg/mL for carbamazepine, and 1-40 microg/mL for zonisamide. Within- and between-run precision studies demonstrated coefficient of variation <10% at all tested concentrations. Other anti-epileptic medications tested did not interfere with the assay. The method is appropriate for determining lamotrigine, carbamazepine and zonisamide serum or plasma concentrations for therapeutic monitoring.

Anticonvulsants - blood

Reproducibility of Results

Chromatography, High Pressure Liquid - methods

Spectrophotometry, Ultraviolet - methods

Humans

Plasma - chemistry

Sensitivity and Specificity

Isoxazoles - blood

Adult

Triazines - blood

Carbamazepine - blood

Details

Title: Subtitle: Simultaneous determination of lamotrigine, zonisamide, and carbamazepine in human plasma by high-performance liquid chromatography
Creators: Elizabeth Greiner-Sosanko - Toxicology and Therapeutic Drug Monitoring Laboratory, Division of Clinical Chemistry, Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
Darla R Lower
Mohamed A Virji
Matthew D Krasowski
Resource Type: Journal article
Publication Details: Biomedical chromatography, Vol.21(3), pp.225-228
DOI: 10.1002/bmc.753
PMID: 17230449
PMCID: PMC2231333
NLM abbreviation: Biomed Chromatogr
ISSN: 0269-3879
eISSN: 1099-0801
Publisher: England
Grant note: K08 GM074238-01A1 / NIGMS NIH HHS K08 GM074238 / NIGMS NIH HHS K08-GM074238-01A1 / NIGMS NIH HHS
Language: English
Date published: 03/2007
Academic Unit: Pathology
Record Identifier: 9984046811002771

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