Journal article
Simultaneous determination of the flavonoids robinin and kaempferol in human breast cancer cells by liquid chromatography-tandem mass spectrometry
Journal of pharmaceutical and biomedical analysis, Vol.55(1), pp.109-113
2011
DOI: 10.1016/j.jpba.2010.12.021
PMID: 21232900
Abstract
An accurate, precise and sensitive method was developed and validated for the simultaneous quantification of the flavonoid glycoside robinin, and its algycone kaempferol in human breast cancer MCF-7 cells. The application of liquid chromatography-tandem mass spectrometry (LC/MS/MS) with a TurboIonspray interface in negative mode under multiple reactions monitoring was investigated. Chromatographic separation was achieved on a C
18 column using a mobile phase consisting of (A) water with 0.025% formic acid and 1
mM ammonium formate and (B) acetonitrile with 0.025% formic acid. Rutin was used as the internal standard for robinin and fisetin as the internal standard for kaempferol. The assay had a limit of detection of 0.1
ng/ml for both compounds when present in cell lysate. The calibration curves were linear from 1 to 250
ng/ml (
r
>
0.999) for each compound. The intra- and inter-day coefficients of variation were less than 10% and intra- and inter-day accuracies were within 11%. This assay was successfully applied in a robinin cellular uptake study to determine the intracellular concentrations of robinin in MCF-7 cells.
Details
- Title: Subtitle
- Simultaneous determination of the flavonoids robinin and kaempferol in human breast cancer cells by liquid chromatography-tandem mass spectrometry
- Creators
- Lia Tsiklauri - Iovel Kutateladze Pharmacochemistry Institute, P. Sarajishvili st 36, Tbilisi 0159, GeorgiaGuohua An - Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, 517 Hochstetter Hall, Amherst, NY 14260-1200, USADonna M Ruszaj - Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, 517 Hochstetter Hall, Amherst, NY 14260-1200, USAMary Alaniya - Iovel Kutateladze Pharmacochemistry Institute, P. Sarajishvili st 36, Tbilisi 0159, GeorgiaEther Kemertelidze - Iovel Kutateladze Pharmacochemistry Institute, P. Sarajishvili st 36, Tbilisi 0159, GeorgiaMarilyn E Morris - Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, 517 Hochstetter Hall, Amherst, NY 14260-1200, USA
- Resource Type
- Journal article
- Publication Details
- Journal of pharmaceutical and biomedical analysis, Vol.55(1), pp.109-113
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jpba.2010.12.021
- PMID
- 21232900
- ISSN
- 0731-7085
- eISSN
- 1873-264X
- Language
- English
- Date published
- 2011
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984065692802771
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