Journal article
Simultaneous loss of multiple differentiated functions in aerial mycelium-negative isolates of streptomycetes
Journal of bacteriology, Vol.137(2), pp.891-899
02/1979
DOI: 10.1128/jb.137.2.891-899.1979
PMCID: PMC218372
PMID: 422514
Abstract
Germination and outgrowth of spores of Streptomyces alboniger, Streptomyces scabies, and Streptomyces violaceus-ruber in the presence of intercalating dyes resulted in a high frequency (2 to 20%) of occurrence of aerial mycelium-negative (Amy-) isolates. Coincident with the appearance of the Amy- trait was the loss of several differentiated functions, including the characteristic pigments and earthy odor of the wild types. All S. alboniger, 27% of S. scabies, and 39% of the S. violaceus-ruber Amy- isolates were arginine auxotrophs. The missing enzyme step was identified as argininosuccinate synthetase by using a sensitive microassay for estimation of enzyme activity. The remainder of the S. scabies and S. violaceus-ruber isolates were prototrophs. In addition, S. alboniger Amy- isolates failed to produce or respond to the stimulator of aerial mycelium formation isolated from the wild type. The Amy- isolates did not revert to either Amy+ of Arg+. The lack of any detectable reversion, coupled with the high frequency of curing, supports the idea that a deletion of genetic material, possibly a plasmid, has occurred.
Details
- Title: Subtitle
- Simultaneous loss of multiple differentiated functions in aerial mycelium-negative isolates of streptomycetes
- Creators
- P A RedshawP A McCannM A PentellaB M Pogell
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.137(2), pp.891-899
- DOI
- 10.1128/jb.137.2.891-899.1979
- PMID
- 422514
- PMCID
- PMC218372
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Language
- English
- Date published
- 02/1979
- Academic Unit
- Central Control Hygienic Laboratory; Epidemiology
- Record Identifier
- 9984214843902771
Metrics
8 Record Views