Journal article
Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy
Biotechnology and bioengineering, Vol.55(1), pp.11-15
07/05/1997
DOI: 10.1002/(SICI)1097-0290(19970705)55:1<11::AID-BIT2>3.0.CO;2-#
PMID: 18636439
Abstract
The purpose of this study was to develop non‐invasive techniques to monitor the composition of cell culture media in insect cell bioreactors. Such a monitor could be used in conjunction with a fed‐batch feeding scheme to ensure that cells are maintained in an optimal environment for growth and protein production. Glucose and glutamine concentrations in an insect cell culture bioreactor were determined off‐line with near‐infrared (NIR) absorption spectroscopy. Spectra were collected from 5000 to 4000 cm−1 with a 1.5‐mm optical path length. Partial least squares (PLS) regression was applied to correlate the collected spectra with the concentration of the desired analytes. Under the culture conditions evaluated here, glucose and glutamine concentrations ranged from 38 to 55 mM and from 3 to 13 mM, respectively. Accurate measurements of glucose and glutamine in insect cell culture samples were possible over these entire ranges. The standard error of prediction (SEP) and mean percent error (MPE) for glutamine were 0.52 mM and 5.3%, respectively. Glucose could be measured with an SEP of 1.30 mM and an MPE of 2.3%. These levels of error are quite low considering the changing complexity of the growth media due to the shifting levels of amino acids, carbohydrates, yeastolate, proteins, and cell debris. This study represents an important step in the development of noninvasive on‐line monitoring devices for cell culture bioreactors. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 11–15, 1997.
Details
- Title: Subtitle
- Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy
- Creators
- Mark R RileyMartin RhielXiangji ZhouMark A ArnoldDavid W Murhammer
- Resource Type
- Journal article
- Publication Details
- Biotechnology and bioengineering, Vol.55(1), pp.11-15
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- DOI
- 10.1002/(SICI)1097-0290(19970705)55:1<11::AID-BIT2>3.0.CO;2-#
- PMID
- 18636439
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- Number of pages
- 5
- Grant note
- NASA (WAG‐9‐824) American Cyanamid
- Language
- English
- Date published
- 07/05/1997
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Chemistry; Chemical and Biochemical Engineering
- Record Identifier
- 9984003476802771
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