Single-cell cytokine analysis of gamma delta T cell responses to nonpeptide mycobacterial antigens

Veronica E García; Peter A Sieling; JianHua Gong; Peter F Barnes; Koichi Uyemura; Yoshi Tanaka; Barry R Bloom; Craig T Morita; Robert L Modlin

doi:10.4049/jimmunol.159.3.1328

Back

Single-cell cytokine analysis of gamma delta T cell responses to nonpeptide mycobacterial antigens

Journal article

Peer reviewed

Single-cell cytokine analysis of gamma delta T cell responses to nonpeptide mycobacterial antigens

Veronica E García, Peter A Sieling, JianHua Gong, Peter F Barnes, Koichi Uyemura, Yoshi Tanaka, Barry R Bloom, Craig T Morita and Robert L Modlin

The Journal of immunology (1950), Vol.159(3), pp.1328-1335

08/01/1997

DOI: 10.4049/jimmunol.159.3.1328

PMID: 9233629

View Online

Abstract

TCR gamma delta T cells are considered important in the rapid immune response to intracellular infection. We investigated the early response of peripheral blood gamma delta T cells to the nonpeptide Ag isopentenyl pyrophosphate and to its synthetic analogue ethyl pyrophosphate. In healthy donors, an increase in the number of gamma delta T cells was detected as soon as 4 days after stimulation with the nonpeptide Ags. Single-cell analysis of cytokine production was performed by intracellular staining of IFN-gamma and IL-4. gamma delta T cells were found to rapidly expand and produce IFN-gamma in response to nonpeptide Ags. Furthermore, IL-12 augmented the IFN-gamma response. In contrast, gamma delta T cells from the majority of HIV+ donors did not expand or express IFN-gamma in response to nonpeptide Ags, even in the presence of IL-12. These findings indicate a role for nonpeptide-reactive gamma delta T cells in effective cell-mediated immunity for intracellular pathogens.

T-Lymphocyte Subsets - immunology

Organophosphorus Compounds - immunology

Mycobacterium - immunology

Cytokines - analysis

Humans

Cells, Cultured

Antigens, Bacterial - pharmacology

Receptors, Antigen, T-Cell, gamma-delta - analysis

Lymphocyte Activation - drug effects

T-Lymphocyte Subsets - drug effects

T-Lymphocyte Subsets - metabolism

Hemiterpenes

Organophosphorus Compounds - pharmacology

Kinetics

HIV Seropositivity - immunology

Cytokines - biosynthesis

Interleukin-12 - pharmacology

Details

Title: Subtitle: Single-cell cytokine analysis of gamma delta T cell responses to nonpeptide mycobacterial antigens
Creators: Veronica E García - Division of Dermatology, University of California Los Angeles School of Medicine, 90095, USA
Peter A Sieling
JianHua Gong
Peter F Barnes
Koichi Uyemura
Yoshi Tanaka
Barry R Bloom
Craig T Morita
Robert L Modlin
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.159(3), pp.1328-1335
DOI: 10.4049/jimmunol.159.3.1328
PMID: 9233629
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AR40312 / NIAMS NIH HHS AI36069 / NIAID NIH HHS AI22553 / NIAID NIH HHS
Language: English
Date published: 08/01/1997
Academic Unit: Immunology; Internal Medicine
Record Identifier: 9984094624702771

Metrics

12 Record Views

118 Times Cited - Web of Science