Journal article
Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage
Osteoarthritis and cartilage, Vol.22(9), pp.1318-1326
09/2014
DOI: 10.1016/j.joca.2014.07.002
PMCID: PMC4950515
PMID: 25038490
Abstract
Objective
To date, no approved clinical intervention successfully prevents the progressive degradation of injured articular cartilage that leads to osteoarthritis (OA). Stem/progenitor cell populations within tissues of diarthrodial joint have shown their therapeutic potential in treating OA. However, this potential has not been fully realized due in part to the heterogeneity of these subpopulations. Characterization of clonal populations derived from a single cell may help identify more homogenous stem/progenitor populations within articular cartilage. Moreover, chondrogenic potential of clonal populations from different zones could be further examined to elucidate their differential roles in maintaining articular cartilage homeostasis.
Method
We combined Fluorescence-activated cell sorting (FACS) and clonogenicity screening to identify stem/progenitor cells cloned from single cells. High-efficiency colony-forming cells (HCCs) were isolated, and evaluated for stem/progenitor cell characteristics. HCCs were also isolated from different zones of articular cartilage. Their function was compared by lineage-specific gene expression, and differentiation potential.
Results
A difference in colony-forming efficiency was observed in terms of colony sizes. HCCs were highly clonogenic and multipotent, and overexpressed stem/progenitor cell markers. Also, proliferation and migration associated genes were over-expressed in HCCs. HCCs showed zonal differences with deep HCCs more chondrogenic and osteogenic than superficial HCCs.
Conclusion
Our approach is a simple yet practical way to identify homogeneous stem/progenitor cell populations with clonal origin. The discovery of progenitor cells demonstrates the intrinsic self-repairing potential of articular cartilage. Differences in differentiation potential may represent the distinct roles of superficial and deep zone stem/progenitor cells in the maintenance of articular cartilage homeostasis.
Details
- Title: Subtitle
- Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage
- Creators
- Yin Yu - Department of Orthopaedics and Rehabilitation, the University of Iowa, Iowa City, IAHongjun Zheng - Department of Orthopaedics and Rehabilitation, the University of Iowa, Iowa City, IAJoseph A Buckwalter - Department of Orthopaedics and Rehabilitation, the University of Iowa, Iowa City, IAJames A Martin - Department of Orthopaedics and Rehabilitation, the University of Iowa, Iowa City, IA
- Resource Type
- Journal article
- Publication Details
- Osteoarthritis and cartilage, Vol.22(9), pp.1318-1326
- DOI
- 10.1016/j.joca.2014.07.002
- PMID
- 25038490
- PMCID
- PMC4950515
- NLM abbreviation
- Osteoarthritis Cartilage
- ISSN
- 1063-4584
- eISSN
- 1522-9653
- Grant note
- DOI: 10.13039/100000005, name: U.S. Department of Defense, award: W81XWH-10-1-0702a; name: American Arthritis Society
- Language
- English
- Date published
- 09/2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Injury Prevention Research Center
- Record Identifier
- 9984040398302771
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