Journal article
Single-cell transcriptomics reveals cell-type-specific diversification in human heart failure
NATURE CARDIOVASCULAR RESEARCH, Vol.1(3), pp.263-280
03/01/2022
DOI: 10.1038/s44161-022-00028-6
PMID: 35959412
Abstract
Heart failure represents a major cause of morbidity and mortality worldwide. Single-cell transcriptomics have revolutionized our understanding of cell composition and associated gene expression. Through integrated analysis of single-cell and single-nucleus RNA-sequencing data generated from 27 healthy donors and 18 individuals with dilated cardiomyopathy, here we define the cell composition of the healthy and failing human heart. We identify cell-specific transcriptional signatures associated with age and heart failure and reveal the emergence of disease-associated cell states. Notably, cardiomyocytes converge toward common disease-associated cell states, whereas fibroblasts and myeloid cells undergo dramatic diversification. Endothelial cells and pericytes display global transcriptional shifts without changes in cell complexity. Collectively, our findings provide a comprehensive analysis of the cellular and transcriptomic landscape of human heart failure, identify cell type-specific transcriptional programs and disease-associated cell states and establish a valuable resource for the investigation of human heart failure.
Koenig et al. present integrated single-cell and single-nucleus RNA-sequencing data of cardiac samples obtained from 27 healthy donors and 18 individuals with dilated cardiomyopathy. This extensive resource provides insights on cell composition and gene expression changes driven by the disease status, sex or age of the patients.
Details
- Title: Subtitle
- Single-cell transcriptomics reveals cell-type-specific diversification in human heart failure
- Creators
- Andrew L. Koenig - Washington University in St. LouisIrina Shchukina - Washington University in St. LouisJunedh Amrute - Washington University in St. LouisPrabhakar S. Andhey - Washington University in St. LouisKonstantin Zaitsev - Washington University in St. LouisLulu Lai - Washington University in St. LouisGeetika Bajpai - Washington University in St. LouisAndrea Bredemeyer - Washington University in St. LouisGabriella Smith - Washington University in St. LouisCameran Jones - Washington University in St. LouisEmily Terrebonne - Washington University in St. LouisStacey L. Rentschler - Washington University in St. LouisMaxim N. Artyomov - Washington University in St. LouisKory J. Lavine - Washington University in St. Louis
- Resource Type
- Journal article
- Publication Details
- NATURE CARDIOVASCULAR RESEARCH, Vol.1(3), pp.263-280
- DOI
- 10.1038/s44161-022-00028-6
- PMID
- 35959412
- ISSN
- 2731-0590
- eISSN
- 2731-0590
- Publisher
- Springer Nature
- Number of pages
- 39
- Grant note
- 3770 / Aging Biology Foundation P30DK052574 / National Institute of Diabetes and Digestive and Kidney Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) R01 HL138466; R01 HL139714; R01 HL151078; R35 HL161185; T32 HL134635; T32 HL125241 / American Heart Association (American Heart Association, Inc.); American Heart Association CDI-CORE-2015-505; CDI-CORE-2019-813 / Children's Discovery Institute of Washington University 8038-88 / St. Louis Children's Hospital Foundation for Barnes-Jewish Hospital R01HL139714; R01HL138466; T32HL125241; R01HL151078; T32HL134635; R35HL161185 / National Heart Lung and Blood Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) Leducq Foundation Network; Leducq Foundation 20CVD02; 1014782 / National Heart, Lung, and Blood Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) Foundation of Barnes-Jewish Hospital
- Language
- English
- Date published
- 03/01/2022
- Academic Unit
- Stead Family Department of Pediatrics
- Record Identifier
- 9985161457202771
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