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Single dose of glycoengineered anti-CD19 antibody (MEDI551) disrupts experimental autoimmune encephalomyelitis by inhibiting pathogenic adaptive immune responses in the bone marrow and spinal cord while preserving peripheral regulatory mechanisms
Journal article   Peer reviewed

Single dose of glycoengineered anti-CD19 antibody (MEDI551) disrupts experimental autoimmune encephalomyelitis by inhibiting pathogenic adaptive immune responses in the bone marrow and spinal cord while preserving peripheral regulatory mechanisms

Ding Chen, Monica Blazek, Sara Ireland, Sterling Ortega, Xiangmei Kong, Anouk Meeuwissen, Ann Stowe, Laura Carter, Yue Wang, Ronald Herbst, …
The Journal of immunology (1950), Vol.193(10), pp.4823-4832
11/15/2014
DOI: 10.4049/jimmunol.1401478
PMCID: PMC4373313
PMID: 25281717

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Abstract

Plasma cells and the autoreactive Abs they produce are suspected to contribute to the pathogenesis of multiple sclerosis, but recent attempts to target these components of humoral immunity have failed. MEDI551, an anti-CD19 Ab that depletes mature B cells including plasma cells may offer a compelling alternative that reduces pathogenic adaptive immune responses while sparing regulatory mechanisms. Indeed, our data demonstrate that a single dose of MEDI551, given before or during ongoing experimental autoimmune encephalomyelitis, disrupts development of the disease. Leukocyte infiltration into the spinal cord is significantly reduced, as well as short-lived and long-lived autoreactive CD138(+) plasma cells in the spleen and bone marrow, respectively. In addition, potentially protective CD1d(hi)CD5(+) regulatory B cells show resistance to depletion, and myelin-specific Foxp3(+) regulatory T cells are expanded. Taken together, these results demonstrate that MEDI551 disrupts experimental autoimmune encephalomyelitis by inhibiting multiple proinflammatory components whereas preserving regulatory populations.
Forkhead Transcription Factors - immunology Bone Marrow - immunology Spleen - immunology Antigens, CD1d - immunology Encephalomyelitis, Autoimmune, Experimental - immunology Male Antigens, CD19 - genetics Lymphocyte Depletion T-Lymphocytes, Regulatory - pathology B-Lymphocytes, Regulatory - pathology Plasma Cells - pathology T-Lymphocytes, Regulatory - immunology Encephalomyelitis, Autoimmune, Experimental - chemically induced Spinal Cord - pathology Protein Engineering Antigens, CD1d - genetics Encephalomyelitis, Autoimmune, Experimental - genetics Spleen - pathology Antigens, CD19 - immunology Gene Expression Signal Transduction Cell Survival Myelin-Oligodendrocyte Glycoprotein Mice, Transgenic B-Lymphocytes, Regulatory - immunology Forkhead Transcription Factors - genetics Antibodies - pharmacology Spinal Cord - immunology Adaptive Immunity - drug effects Animals Bone Marrow - pathology Encephalomyelitis, Autoimmune, Experimental - prevention & control Mice Plasma Cells - immunology

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